{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hoover EM"],"funding":["NCATS NIH HHS","NEI NIH HHS","NIA NIH HHS","NIAID NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["109044"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8095351"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["350"],"pubmed_abstract":["<h4>Background</h4>The regulation of cerebral blood flow is critical for normal brain functioning, and many physiological and pathological conditions can have long-term impacts on cerebral blood flow. However, minimally invasive tools to study chronic changes in animal models are limited.<h4>New method</h4>We developed a minimally invasive surgical technique (cyanoacrylate skull, CAS) allowing us to image cerebral blood flow longitudinally through the intact mouse skull using laser speckle imaging.<h4>Results</h4>With CAS we were able to detect acute changes in cerebral blood flow induced by hypercapnic challenge. We were also able to image cerebral blood flow dynamics with laser speckle imaging for over 100 days. Furthermore, the relative cerebral blood flow remained stable in mice from 3"],"journal":["Journal of neuroscience methods"],"pubmed_title":["Transcranial chronic optical access to longitudinally measure cerebral blood flow."],"pmcid":["PMC8095351"],"funding_grant_id":["R01 EY029490","T32 GM008620","R21 AG066000","T32 AI060573","R01 AI120846","TL1 TR001415"],"pubmed_authors":["Lodoen MB","Figueroa Velez DX","Choi B","Gandhi SP","Crouzet C","Hoover EM","Bordas JM"],"additional_accession":[]},"is_claimable":false,"name":"Transcranial chronic optical access to longitudinally measure cerebral blood flow.","description":"<h4>Background</h4>The regulation of cerebral blood flow is critical for normal brain functioning, and many physiological and pathological conditions can have long-term impacts on cerebral blood flow. However, minimally invasive tools to study chronic changes in animal models are limited.<h4>New method</h4>We developed a minimally invasive surgical technique (cyanoacrylate skull, CAS) allowing us to image cerebral blood flow longitudinally through the intact mouse skull using laser speckle imaging.<h4>Results</h4>With CAS we were able to detect acute changes in cerebral blood flow induced by hypercapnic challenge. We were also able to image cerebral blood flow dynamics with laser speckle imaging for over 100 days. Furthermore, the relative cerebral blood flow remained stable in mice from 3","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-27T01:42:57.762Z","creation":"2025-04-06T18:21:27.577Z"},"accession":"S-EPMC8095351","cross_references":{"pubmed":["33340556"],"doi":["10.1016/j.jneumeth.2020.109044"]}}