{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Andersson M"],"funding":["Science for Life Laboratory","Vetenskapsrådet","European Research Council","Knut och Alice Wallenbergs Stiftelse","Uppsala Universitet"],"pagination":["1694-1705"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8095708"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(9)"],"pubmed_abstract":["This work describes a programmable heat-stage compatible with in situ microscopy for the accurate provision of spatiotemporally defined temperatures to different microfluidic devices. The heat-stage comprises an array of integrated thin-film Joule heaters and resistance temperature detectors (RTDs). External programming of the heat-stage is provided by a custom software program connected to temperature controllers and heater-sensor pairs. Biologically relevant (20-40 °C) temperature profiles can be supplied to cells within microfluidic devices as spatial gradients (0.5-1.5 °C mm-1) or in a time-varying approach via e.g. step-wise or sinusoidally varying profiles with negligible temperature over-shoot. Demonstration of the device is achieved by exposing two strains of the coral symbiont Sym"],"journal":["Lab on a chip"],"pubmed_title":["A microscopy-compatible temperature regulation system for single-cell phenotype analysis - demonstrated by thermoresponse mapping of microalgae."],"pmcid":["PMC8095708"],"funding_grant_id":["757444","2019-04401","WAF 2016.0112"],"pubmed_authors":["Tenje M","Johansson S","Xiao L","Behrendt L","Bergman H","Andersson M"],"additional_accession":[]},"is_claimable":false,"name":"A microscopy-compatible temperature regulation system for single-cell phenotype analysis - demonstrated by thermoresponse mapping of microalgae.","description":"This work describes a programmable heat-stage compatible with in situ microscopy for the accurate provision of spatiotemporally defined temperatures to different microfluidic devices. The heat-stage comprises an array of integrated thin-film Joule heaters and resistance temperature detectors (RTDs). External programming of the heat-stage is provided by a custom software program connected to temperature controllers and heater-sensor pairs. Biologically relevant (20-40 °C) temperature profiles can be supplied to cells within microfluidic devices as spatial gradients (0.5-1.5 °C mm-1) or in a time-varying approach via e.g. step-wise or sinusoidally varying profiles with negligible temperature over-shoot. Demonstration of the device is achieved by exposing two strains of the coral symbiont Sym","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-07-16T08:54:41.879Z","creation":"2022-02-10T10:01:47.476Z"},"accession":"S-EPMC8095708","cross_references":{"pubmed":["33949404"],"doi":["10.1039/d0lc01288b"]}}