{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(7)"],"submitter":["Tastanova A"],"pubmed_abstract":["Real-time RT-PCR remains a gold standard in the detection of various viral diseases. In the coronavirus 2019 pandemic, multiple RT-PCR-based tests were developed to screen for viral infection. As an emergency response to increasing testing demand, we established a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) PCR diagnostics platform for which we compared different commercial and in-house RT-PCR protocols. Four commercial, one customized, and one in-house RT-PCR protocols were evaluated with 92 SARS-CoV-2-positive and 92 SARS-CoV-2-negative samples. Furthermore, economical and practical characteristics of these protocols were compared. In addition, a highly sensitive digital droplet PCR (ddPCR) method was developed, and application of RT-PCR and ddPCR methods on SARS-CoV-2 e"],"journal":["The Journal of molecular diagnostics : JMD"],"pagination":["796-804"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8096526"],"repository":["biostudies-literature"],"pubmed_title":["A Comparative Study of Real-Time RT-PCR-Based SARS-CoV-2 Detection Methods and Its Application to Human-Derived and Surface Swabbed Material."],"pmcid":["PMC8096526"],"pubmed_authors":["Cheng PF","Dzung A","Levesque MP","Bellini E","Nobbe S","Lienhard R","Duda A","Tastanova A","Johansen P","Bosshard PP","Stoffel CI"],"additional_accession":[]},"is_claimable":false,"name":"A Comparative Study of Real-Time RT-PCR-Based SARS-CoV-2 Detection Methods and Its Application to Human-Derived and Surface Swabbed Material.","description":"Real-time RT-PCR remains a gold standard in the detection of various viral diseases. In the coronavirus 2019 pandemic, multiple RT-PCR-based tests were developed to screen for viral infection. As an emergency response to increasing testing demand, we established a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) PCR diagnostics platform for which we compared different commercial and in-house RT-PCR protocols. Four commercial, one customized, and one in-house RT-PCR protocols were evaluated with 92 SARS-CoV-2-positive and 92 SARS-CoV-2-negative samples. Furthermore, economical and practical characteristics of these protocols were compared. In addition, a highly sensitive digital droplet PCR (ddPCR) method was developed, and application of RT-PCR and ddPCR methods on SARS-CoV-2 e","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-05-29T22:18:03.908Z","creation":"2025-05-29T22:18:03.908Z"},"accession":"S-EPMC8096526","cross_references":{"pubmed":["33962053"],"doi":["10.1016/j.jmoldx.2021.04.009"]}}