{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["67"],"submitter":["Klausberger M"],"funding":["BOKU","WWTF","Ludwig Boltzmann Institute for Experimental and Clinical Traumatology","OeAD","FWO"],"pubmed_abstract":["<h4>Background</h4>Antibody tests are essential tools to investigate humoral immunity following SARS-CoV-2 infection or vaccination. While first-generation antibody tests have primarily provided qualitative results, accurate seroprevalence studies and tracking of antibody levels over time require highly specific, sensitive and quantitative test setups.<h4>Methods</h4>We have developed two quantitative, easy-to-implement SARS-CoV-2 antibody tests, based on the spike receptor binding domain and the nucleocapsid protein. Comprehensive evaluation of antigens from several biotechnological platforms enabled the identification of superior antigen designs for reliable serodiagnostic. Cut-off modelling based on unprecedented large and heterogeneous multicentric validation cohorts allowed us to defi"],"journal":["EBioMedicine"],"pagination":["103348"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8099623"],"repository":["biostudies-literature"],"pubmed_title":["A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests."],"pmcid":["PMC8099623"],"pubmed_authors":["Strassl R","Wozniak-Knopp G","Borth N","Satzer P","Huber J","De Vos J","Stadler M","Wahrmann M","Stadlmayr G","Griesmacher A","Binder NB","Vierlinger K","Beck J","Binder CJ","Tauer C","Sieghart D","Breyer MK","Striedner G","Hoermann G","Hahn R","Jungbauer A","Koppl C","Gludovacz E","Laurent E","Lingg N","Leitner G","Hofner M","Vavra U","Manhart G","Klausberger M","Milchram L","Strobl F","Gerner W","Haslacher H","Grabherr R","Egger M","Duerkop M","Weiss G","Eskandary F","Grebien F","Marx N","Holzer B","Bellmann-Weiler R","Aguilar PP","Perkmann T","Hartmann B","Grunwald-Gruber C","Egger AE","Irsara C","Mairhofer J","Mach L","Cserjan-Puschmann M","Weinhausel A","Dieplinger B","Loeffler-Ragg J","Strasser R","Quehenberger P","Maresch D"],"additional_accession":[]},"is_claimable":false,"name":"A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests.","description":"<h4>Background</h4>Antibody tests are essential tools to investigate humoral immunity following SARS-CoV-2 infection or vaccination. While first-generation antibody tests have primarily provided qualitative results, accurate seroprevalence studies and tracking of antibody levels over time require highly specific, sensitive and quantitative test setups.<h4>Methods</h4>We have developed two quantitative, easy-to-implement SARS-CoV-2 antibody tests, based on the spike receptor binding domain and the nucleocapsid protein. Comprehensive evaluation of antigens from several biotechnological platforms enabled the identification of superior antigen designs for reliable serodiagnostic. Cut-off modelling based on unprecedented large and heterogeneous multicentric validation cohorts allowed us to defi","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-07-16T06:16:40.481Z","creation":"2022-02-10T15:46:42.643Z"},"accession":"S-EPMC8099623","cross_references":{"pubmed":["33906067"],"doi":["10.1016/j.ebiom.2021.103348"]}}