{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(1)"],"submitter":["Kleiman E"],"funding":["JSR Life Sciences, LLC"],"pubmed_abstract":["Extracellular adenosine suppresses T cell immunity in the tumor microenvironment and in vitro treatment of memory T cells with adenosine can suppress antigen-mediated memory T cell expansion. We describe utilizing the recall antigen assay platform to screen small molecule drug off-target effects on memory T cell expansion/function using a dosing regimen based on adenosine treatment. As a proof of principle, we show low dose GS-5734, a monophosphoramidate prodrug of an adenosine analog, does not alter memory T cell recall at lower doses whereas toxicity observed at high dose favors antigen-specific memory T cell survival/proliferation over non-specific CD8<sup>+</sup> T cells. Conversely, parent nucleoside GS-441524 at high dosage does not result in cellular toxicity and reduces antigen-spe"],"journal":["Scientific reports"],"pagination":["9561"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8100288"],"repository":["biostudies-literature"],"pubmed_title":["Adenosine-related small molecules show utility of recall antigen assay to screen compounds for off-target effects on memory T cells."],"pmcid":["PMC8100288"],"pubmed_authors":["Kleiman E","Magcase D","Mao B","Daftarian PM","Sierra G","George MV"],"additional_accession":[]},"is_claimable":false,"name":"Adenosine-related small molecules show utility of recall antigen assay to screen compounds for off-target effects on memory T cells.","description":"Extracellular adenosine suppresses T cell immunity in the tumor microenvironment and in vitro treatment of memory T cells with adenosine can suppress antigen-mediated memory T cell expansion. We describe utilizing the recall antigen assay platform to screen small molecule drug off-target effects on memory T cell expansion/function using a dosing regimen based on adenosine treatment. As a proof of principle, we show low dose GS-5734, a monophosphoramidate prodrug of an adenosine analog, does not alter memory T cell recall at lower doses whereas toxicity observed at high dose favors antigen-specific memory T cell survival/proliferation over non-specific CD8<sup>+</sup> T cells. Conversely, parent nucleoside GS-441524 at high dosage does not result in cellular toxicity and reduces antigen-spe","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-08T09:39:49.668Z","creation":"2022-02-10T09:33:20.103Z"},"accession":"S-EPMC8100288","cross_references":{"pubmed":["33953256"],"doi":["10.1038/s41598-021-88965-3"]}}