biostudies-literatureUnknown21(6)Huang Y<i>Carpesium cernuum</i> L. is a traditional medicine primarily used in Southwestern China, and it has been shown to exhibit a range of biological properties, including anti-inflammatory and antitumor activities. Incaspitolide A (IA) is a sesquiterpene isolated from <i>C. cernuum</i> L. The aim of the present study was to investigate the antiproliferative effects of IA on PC-3 prostate cancer cells and determine the underlying mechanism. Results from a Cell Counting Kit-8 assay demonstrated that IA significantly reduced the numbers of viable PC-3 cells in a time and dose-dependent manner. Phase-contrast microscopy revealed that the number and morphology of cells were markedly altered. Hoechst and EdU staining assays showed that IA reduced the proliferation of PC-3 cells. Flow cytometry anaOncology letters477https://www.ebi.ac.uk/biostudies/studies/S-EPMC8100957biostudies-literatureIncaspitolide A isolated from <i>Carpesium cernuum</i> L. inhibits the growth of prostate cancer cells and induces apoptosis via regulation of the PI3K/Akt/xIAP pathway.PMC8100957Huang YGuo HChen MYan CZhang LMao JfalseIncaspitolide A isolated from <i>Carpesium cernuum</i> L. inhibits the growth of prostate cancer cells and induces apoptosis via regulation of the PI3K/Akt/xIAP pathway.<i>Carpesium cernuum</i> L. is a traditional medicine primarily used in Southwestern China, and it has been shown to exhibit a range of biological properties, including anti-inflammatory and antitumor activities. Incaspitolide A (IA) is a sesquiterpene isolated from <i>C. cernuum</i> L. The aim of the present study was to investigate the antiproliferative effects of IA on PC-3 prostate cancer cells and determine the underlying mechanism. Results from a Cell Counting Kit-8 assay demonstrated that IA significantly reduced the numbers of viable PC-3 cells in a time and dose-dependent manner. Phase-contrast microscopy revealed that the number and morphology of cells were markedly altered. Hoechst and EdU staining assays showed that IA reduced the proliferation of PC-3 cells. Flow cytometry ana2021-01-01T00:00:00Z2021 Jun2025-04-21T18:22:16.52Z2025-04-05T17:13:03.748ZS-EPMC81009573396819310.3892/ol.2021.12738