biostudies-literatureCurtis KJNCATS NIH HHSNCI NIH HHS1009-1019https://www.ebi.ac.uk/biostudies/studies/S-EPMC8101488biostudies-literatureUnknown32(10)Metastasis is responsible for over 90% of cancer-related deaths, and bone is the most common site for breast cancer metastasis. Metastatic breast cancer cells home to trabecular bone, which contains hematopoietic and stromal lineage cells in the marrow. As such, it is crucial to understand whether bone or marrow cells enhance breast cancer cell migration toward the tissue. To this end, we quantified the migration of MDA-MB-231 cells toward human bone in two- and three-dimensional (3D) environments. First, we found that the cancer cells cultured on tissue culture plastic migrated toward intact trabecular bone explants at a higher rate than toward marrow-deficient bone or devitalized bone. Leptin was more abundant in conditioned media from the cocultures with intact explants, while higher leMolecular biology of the cellThe effect of marrow secretome and culture environment on the rate of metastatic breast cancer cell migration in two and three dimensions.PMC8101488R33 CA206922TL1 TR002531Romero-Moreno RCurtis KJMitros SFMcNamara LMDynako JALittlepage LEOberman AGWalsh MMartin HMai CNiebur GLZimmer DIAlderfer LThomas SGfalseThe effect of marrow secretome and culture environment on the rate of metastatic breast cancer cell migration in two and three dimensions.Metastasis is responsible for over 90% of cancer-related deaths, and bone is the most common site for breast cancer metastasis. Metastatic breast cancer cells home to trabecular bone, which contains hematopoietic and stromal lineage cells in the marrow. As such, it is crucial to understand whether bone or marrow cells enhance breast cancer cell migration toward the tissue. To this end, we quantified the migration of MDA-MB-231 cells toward human bone in two- and three-dimensional (3D) environments. First, we found that the cancer cells cultured on tissue culture plastic migrated toward intact trabecular bone explants at a higher rate than toward marrow-deficient bone or devitalized bone. Leptin was more abundant in conditioned media from the cocultures with intact explants, while higher le2021-01-01T00:00:00Z2021 May2025-04-19T17:47:04.278Z2022-02-11T05:01:50.607ZS-EPMC81014883368939610.1091/mbc.E19-12-0682