biostudies-literatureWilkie HNational Institute of Allergy and Infectious DiseasesNIAID NIH HHSNational Institutes of Health1503-1511.e3https://www.ebi.ac.uk/biostudies/studies/S-EPMC8102650biostudies-literatureUnknown141(6)Chronic dermatitis is a hallmark of Dedicator of cytokinesis 8 (DOCK8) deficiency. The migration of DOCK8-deficient T cells to the skin and their survival there have been reported to be defective. Surprisingly, we found that Dock8^-/- mice demonstrated an exaggerated contact hypersensitivity (CHS) response to oxazolone with increased ear swelling, T-cell infiltration, and expression of Ifng. To understand the mechanisms of persistent skin inflammation in DOCK8 deficiency, we examined mice with selective deficiency of DOCK8 in T cells or T regulatory cells (Tregs) and found that both have exaggerated CHS. Moreover, oral tolerance to oxazolone, mediated by Tregs, was impaired in Dock8^-/- mice. Transfer of Tregs from oxazolone-sensitized wild-type mice, but not Dock8<sup>The Journal of investigative dermatologyDOCK8 Expression in Regulatory T Cells Maintains their Stability and Limits Contact Hypersensitivity.PMC8102650R01 AI153257USPHS RO1AI114588K08AI114968R01 AI100315K08 AI114968T32 AI0075125T32AI007512Geha RSWilkie HLeyva-Castillo JMJanssen EfalseDOCK8 Expression in Regulatory T Cells Maintains their Stability and Limits Contact Hypersensitivity.Chronic dermatitis is a hallmark of Dedicator of cytokinesis 8 (DOCK8) deficiency. The migration of DOCK8-deficient T cells to the skin and their survival there have been reported to be defective. Surprisingly, we found that Dock8^-/- mice demonstrated an exaggerated contact hypersensitivity (CHS) response to oxazolone with increased ear swelling, T-cell infiltration, and expression of Ifng. To understand the mechanisms of persistent skin inflammation in DOCK8 deficiency, we examined mice with selective deficiency of DOCK8 in T cells or T regulatory cells (Tregs) and found that both have exaggerated CHS. Moreover, oral tolerance to oxazolone, mediated by Tregs, was impaired in Dock8^-/- mice. Transfer of Tregs from oxazolone-sensitized wild-type mice, but not Dock8<sup>2021-01-01T00:00:00Z2021 Jun2025-04-29T11:29:24.571Z2025-04-29T11:29:24.571ZS-EPMC81026503317116910.1016/j.jid.2020.09.027