biostudies-literatureBarbosa MFundação para a Ciência e a Tecnologia634355https://www.ebi.ac.uk/biostudies/studies/S-EPMC8103001biostudies-literatureUnknown9Reactive astrocytes in Amyotrophic Lateral Sclerosis (ALS) change their molecular expression pattern and release toxic factors that contribute to neurodegeneration and microglial activation. We and others identified a dysregulated inflammatory miRNA profile in ALS patients and in mice models suggesting that they represent potential targets for therapeutic intervention. Such cellular miRNAs are known to be released into the secretome and to be carried by small extracellular vesicles (sEVs), which may be harmful to recipient cells. Thus, ALS astrocyte secretome may disrupt cell homeostasis and impact on ALS pathogenesis. Previously, we identified a specific aberrant signature in the cortical brain of symptomatic SOD1-G93A (mSOD1) mice, as well as in astrocytes isolated from the same region oFrontiers in cell and developmental biologyRecovery of Depleted miR-146a in ALS Cortical Astrocytes Reverts Cell Aberrancies and Prevents Paracrine Pathogenicity on Microglia and Motor Neurons.PMC8103001SFRH/BD/102718/2014PTDC/MED-NEU/31395/2017UIDB/04138/2020PD/BD/150342/2019SFRH/BPD/76590/2011SFRH/BD/129586/2017UIDP/04138/2020Moreira RBarbosa MGomes CCarvalho LAGoncalves-Ribeiro JVaz SHVaz ARPina CCBrites DSequeira CfalseRecovery of Depleted miR-146a in ALS Cortical Astrocytes Reverts Cell Aberrancies and Prevents Paracrine Pathogenicity on Microglia and Motor Neurons.Reactive astrocytes in Amyotrophic Lateral Sclerosis (ALS) change their molecular expression pattern and release toxic factors that contribute to neurodegeneration and microglial activation. We and others identified a dysregulated inflammatory miRNA profile in ALS patients and in mice models suggesting that they represent potential targets for therapeutic intervention. Such cellular miRNAs are known to be released into the secretome and to be carried by small extracellular vesicles (sEVs), which may be harmful to recipient cells. Thus, ALS astrocyte secretome may disrupt cell homeostasis and impact on ALS pathogenesis. Previously, we identified a specific aberrant signature in the cortical brain of symptomatic SOD1-G93A (mSOD1) mice, as well as in astrocytes isolated from the same region o2021-01-01T00:00:00Z20212026-05-07T22:43:04.428Z2022-02-10T09:42:58.419ZS-EPMC81030013396892310.3389/fcell.2021.634355