biostudies-literatureYang FNIAID NIH HHSFoundation for the National Institutes of HealthNational Natural Science Foundation of China241https://www.ebi.ac.uk/biostudies/studies/S-EPMC8103607biostudies-literatureUnknown14(1)<h4>Background</h4>Transmission-blocking vaccine (TBV) is a promising strategy for malaria elimination. It is hypothesized that mixing or fusing two antigens targeting different stages of sexual development may provide higher transmission-blocking activity than these antigens used individually.<h4>Methods</h4>A chimeric protein composed of fragments of Pbg37 and PSOP25 was designed and expressed the recombinant protein in Escherichia coli Rosetta-gami B (DE3). After immunizing mice with individual recombinant proteins Pbg37 and PSOP25, mixed proteins (Pbg37+PSOP25), or the fusion protein (Pbg37-PSOP25), the antibody titers of individual sera were analyzed by ELISA. IFA and Western blot were performed to test the reactivity of the antisera with the native proteins in the parasite. The transParasites & vectorsEvaluation of two sexual-stage antigens as bivalent transmission-blocking vaccines in rodent malaria.PMC8103607U19AI08967231900674R01AI150533R01 AI150533Qiu YCui LCao YZheng WJin YYang FLiu FYu XWu YfalseEvaluation of two sexual-stage antigens as bivalent transmission-blocking vaccines in rodent malaria.<h4>Background</h4>Transmission-blocking vaccine (TBV) is a promising strategy for malaria elimination. It is hypothesized that mixing or fusing two antigens targeting different stages of sexual development may provide higher transmission-blocking activity than these antigens used individually.<h4>Methods</h4>A chimeric protein composed of fragments of Pbg37 and PSOP25 was designed and expressed the recombinant protein in Escherichia coli Rosetta-gami B (DE3). After immunizing mice with individual recombinant proteins Pbg37 and PSOP25, mixed proteins (Pbg37+PSOP25), or the fusion protein (Pbg37-PSOP25), the antibody titers of individual sera were analyzed by ELISA. IFA and Western blot were performed to test the reactivity of the antisera with the native proteins in the parasite. The trans2021-01-01T00:00:00Z2021 May2025-04-04T20:36:00.248Z2025-04-04T20:36:00.248ZS-EPMC81036073396267110.1186/s13071-021-04743-0