{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Su X"],"funding":["Hunan Provincial Natural Science Foundation of China","Returnee Program of Anhui People’s Society Office","Key Natural Science Research Projects in Anhui Universities","Anhui Province Natural Science Foundation of China","Key Project of Scientific Research Fund of Anhui University of Chinese Medicine"],"pagination":["9810"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8105318"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally. Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus. An L<sub>9</sub>(3<sup>4</sup>) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use. The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly. Platycodin D and total polysaccharide content of calli was found higher than that of leaves. While, platycoside"],"journal":["Scientific reports"],"pubmed_title":["A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis."],"pmcid":["PMC8105318"],"funding_grant_id":["Grant No. 2018zrzd05","Grant No. KJ2019A0453","Grant No. 1908085MH268","Grant No. 2018JJ3008","Grant No. DT18100035"],"pubmed_authors":["Yu N","Cao M","Wu L","Han L","Guo X","Meng F","Liu Y","Gui S","Peng D","Jiang W","Xing S","Su X","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.","description":"Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally. Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus. An L<sub>9</sub>(3<sup>4</sup>) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use. The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly. Platycodin D and total polysaccharide content of calli was found higher than that of leaves. While, platycoside","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-01T07:38:56.476Z","creation":"2025-04-05T00:15:58.6Z"},"accession":"S-EPMC8105318","cross_references":{"pubmed":["33963244"],"doi":["10.1038/s41598-021-89294-1"]}}