biostudies-literatureGauthier AAgence Nationale de la Recherche1141-1150https://www.ebi.ac.uk/biostudies/studies/S-EPMC8108418biostudies-literatureUnknown8(5)We compared Simoa^TM and Ella^TM immunoassays to assess serum neurofilament-light chain levels in 203 multiple sclerosis patients from the OFSEP HD study. There was a strong correlation (ρ = 0.86, p < 0.0001) between both platforms. The Ella^TM instrument overestimated values by 17%, but as the data were linear (p = 0.57), it was possible to apply a correction factor to Ella^TM results. As for Simoa^TM , serum neurofilament-light chain levels measured by Ella^TM were correlated with age and EDSS and were significantly higher in active multiple sclerosis, suggesting that these assays are equivalent and can be used in routine clinical practice.Annals of clinical and translational neurologyComparison of Simoa^TM and Ella^TM to assess serum neurofilament-light chain in multiple sclerosis.PMC8108418ANR‐10‐COHO‐002Perret MCastelnovo GDebouverie MLebrun-Frenay CBrocard GBerger EGout OMoreau TGauthier ACasey RDe Seze JOFSEP InvestigatorsCasez OClavelou PTrouillet-Assant SVukusic SViel SLaplaud DAZephir HLaurent-Chabalier SEdan GRuet AThouvenot ELombard CfalseComparison of Simoa^TM and Ella^TM to assess serum neurofilament-light chain in multiple sclerosis.We compared Simoa^TM and Ella^TM immunoassays to assess serum neurofilament-light chain levels in 203 multiple sclerosis patients from the OFSEP HD study. There was a strong correlation (ρ = 0.86, p < 0.0001) between both platforms. The Ella^TM instrument overestimated values by 17%, but as the data were linear (p = 0.57), it was possible to apply a correction factor to Ella^TM results. As for Simoa^TM , serum neurofilament-light chain levels measured by Ella^TM were correlated with age and EDSS and were significantly higher in active multiple sclerosis, suggesting that these assays are equivalent and can be used in routine clinical practice.2021-01-01T00:00:00Z2021 May2025-04-06T23:53:35.315Z2025-04-04T14:55:25.659ZS-EPMC81084183383065010.1002/acn3.51355