biostudies-literatureUnknown22(1)Huang CFCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of CanadaTürkiye Bilimsel ve Teknolojik Araştirma KurumuKWF Kankerbestrijding<h4>Background</h4>Androgen receptor (AR) is critical to the initiation, growth, and progression of prostate cancer. Once activated, the AR binds to cis-regulatory enhancer elements on DNA that drive gene expression. Yet, there are 10-100× more binding sites than differentially expressed genes. It is unclear how or if these excess binding sites impact gene transcription.<h4>Results</h4>To characterize the regulatory logic of AR-mediated transcription, we generated a locus-specific map of enhancer activity by functionally testing all common clinical AR binding sites with Self-Transcribing Active Regulatory Regions sequencing (STARRseq). Only 7% of AR binding sites displayed androgen-dependent enhancer activity. Instead, the vast majority of AR binding sites were either inactive or constitutGenome biology149https://www.ebi.ac.uk/biostudies/studies/S-EPMC8112059biostudies-literatureFunctional mapping of androgen receptor enhancer activity.PMC8112059van der Poel HAltintas UBHach FHuang CFHoogstraat MStelloo SGleave MEBergman AMOzturan DHu EFeng FYZwart WSar FLack NACollins CMorova TLe Bihan SLingadahalli SGokbayrak BYu IPLLinder SSaffarzadeh MMcConeghy BEmberly EfalseFunctional mapping of androgen receptor enhancer activity.<h4>Background</h4>Androgen receptor (AR) is critical to the initiation, growth, and progression of prostate cancer. Once activated, the AR binds to cis-regulatory enhancer elements on DNA that drive gene expression. Yet, there are 10-100× more binding sites than differentially expressed genes. It is unclear how or if these excess binding sites impact gene transcription.<h4>Results</h4>To characterize the regulatory logic of AR-mediated transcription, we generated a locus-specific map of enhancer activity by functionally testing all common clinical AR binding sites with Self-Transcribing Active Regulatory Regions sequencing (STARRseq). Only 7% of AR binding sites displayed androgen-dependent enhancer activity. Instead, the vast majority of AR binding sites were either inactive or constitut2021-01-01T00:00:00Z2021 May2026-05-09T09:48:20.076Z2025-04-03T21:33:41.185ZS-EPMC81120593397562710.1186/s13059-021-02339-6