biostudies-literatureHoll EKNIAID NIH HHSU.S. Department of DefenseNational Cancer InstituteNCI NIH HHS1772-1781https://www.ebi.ac.uk/biostudies/studies/S-EPMC8116567biostudies-literatureUnknown29(5)Tumor cells release nucleic acid-containing proinflammatory complexes, termed nucleic acid-containing damage-associated molecular patterns (NA DAMPs), passively upon death and actively during stress. NA DAMPs activate pattern recognition receptors on cells in the tumor microenvironment leading to prolonged and intensified inflammation that potentiates metastasis. No strategy exists to control endogenous or therapy-induced inflammation in cancer patients. We discovered that the generation 3.0 polyamidoamine dendrimer (PAMAM-G3) scavenges NA DAMPs and mitigates their proinflammatory effects. In this study, we tested if the nucleic acid scavenger (NAS) PAMAM-G3 reduces lung metastasis in murine models of breast cancer. Our data indicate that PAMAM-G3 treatment decreases cell-free DNA levels aMolecular therapy : the journal of the American Society of Gene TherapyControlling cancer-induced inflammation with a nucleic acid scavenger prevents lung metastasis in murine models of breast cancer.PMC8116567T32 CA093245T32 AI141342Holl EKSullenger BBoczkowski DNair SKFrazier VLanda KfalseControlling cancer-induced inflammation with a nucleic acid scavenger prevents lung metastasis in murine models of breast cancer.Tumor cells release nucleic acid-containing proinflammatory complexes, termed nucleic acid-containing damage-associated molecular patterns (NA DAMPs), passively upon death and actively during stress. NA DAMPs activate pattern recognition receptors on cells in the tumor microenvironment leading to prolonged and intensified inflammation that potentiates metastasis. No strategy exists to control endogenous or therapy-induced inflammation in cancer patients. We discovered that the generation 3.0 polyamidoamine dendrimer (PAMAM-G3) scavenges NA DAMPs and mitigates their proinflammatory effects. In this study, we tested if the nucleic acid scavenger (NAS) PAMAM-G3 reduces lung metastasis in murine models of breast cancer. Our data indicate that PAMAM-G3 treatment decreases cell-free DNA levels a2021-01-01T00:00:00Z2021 May2025-04-04T10:16:41.728Z2025-04-04T10:16:41.728ZS-EPMC81165673334805510.1016/j.ymthe.2020.12.026