{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tessier SN"],"funding":["Massachusetts General Hospital","American Heart Association","Division of Engineering Education and Centers","American Cancer Society","Wang Family Foundation","NHLBI NIH HHS","National Heart, Lung, and Blood Institute","Harvard Medical School","National Cancer Institute","NCI NIH HHS"],"pagination":["e0251290"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8118530"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(5)"],"pubmed_abstract":["Extracellular vesicles (EVs) have emerged as promising candidates in biomarker discovery and diagnostics. Protected by the lipid bilayer, the molecular content of EVs in diverse biofluids are protected from RNases and proteases in the surrounding environment that may rapidly degrade targets of interests. Nonetheless, cryopreservation of EV-containing samples to -80°C may expose the lipid bilayer to physical and biological stressors which may result in cryoinjury and contribute to changes in EV yield, function, or molecular cargo. In the present work, we systematically evaluate the effect of cryopreservation at -80°C for a relatively short duration of storage (up to 12 days) on plasma- and media-derived EV particle count and/or RNA yield/quality, as compared to paired fresh controls. On ave"],"journal":["PloS one"],"pubmed_title":["Isolation of intact extracellular vesicles from cryopreserved samples."],"pmcid":["PMC8118530"],"funding_grant_id":["K99 HL143149","1R01CA226871-01A1","Claflin Distinguished Scholar Award","R01 CA226871","R00 HL143149","1941543","F32 CA236417","132030-RSG-18-108-01-TBG","R01 HL157803","18CDA34110049","Eleanor and Miles Shore Fellowship","K99/R00 HL143149"],"pubmed_authors":["Bookstaver LD","Stannard CJ","Angpraseuth C","Marques B","Rabe DC","Ho UK","Reategui E","Aldikacti B","Tessier SN","Muzikansky A","Stott SL","Toner M"],"additional_accession":[]},"is_claimable":false,"name":"Isolation of intact extracellular vesicles from cryopreserved samples.","description":"Extracellular vesicles (EVs) have emerged as promising candidates in biomarker discovery and diagnostics. Protected by the lipid bilayer, the molecular content of EVs in diverse biofluids are protected from RNases and proteases in the surrounding environment that may rapidly degrade targets of interests. Nonetheless, cryopreservation of EV-containing samples to -80°C may expose the lipid bilayer to physical and biological stressors which may result in cryoinjury and contribute to changes in EV yield, function, or molecular cargo. In the present work, we systematically evaluate the effect of cryopreservation at -80°C for a relatively short duration of storage (up to 12 days) on plasma- and media-derived EV particle count and/or RNA yield/quality, as compared to paired fresh controls. On ave","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-04T23:17:16.217Z","creation":"2025-04-04T23:17:16.217Z"},"accession":"S-EPMC8118530","cross_references":{"pubmed":["33983964"],"doi":["10.1371/journal.pone.0251290"]}}