{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stephenson E"],"funding":["Cancer Research UK","Action Medical Research","Versus Arthritis","Medical Research Council","National Institute for Health Research (NIHR)","Rosetrees","Wellcome Trust","Academy of Medical Sciences","Biotechnology and Biological Sciences Research Council"],"pagination":["904-916"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8121667"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(5)"],"pubmed_abstract":["Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts (CD16<sup>+</sup>C1QA/B/C<sup>+</sup>) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34<sup>+</sup> hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by ex"],"journal":["Nature medicine"],"pubmed_title":["Single-cell multi-omics analysis of the immune response in COVID-19."],"pmcid":["PMC8121667"],"funding_grant_id":["S_3517","MR/W014556/1","NIHR201394","MC_PC_17230","MR/S036113/1","ACF-2020-01-007","WT107931/Z/15/Z","BB/P002293/1","DRCPGM\\100015","214539/Z/18/Z","107630/Z/15/Z","WT211276/Z/18/Z","211153/Z/18/Z","ACF-2018-01-004","MR/P008801/1","ACF-2018-01-001","211276/Z/18/Z","CL-2013-01-003","SGL015\\1021","204721/Z/16/Z","BB/V006738/1","MR/R015635/1","21777","M944","2779","2911","CL-2015-01-002","CL-2017-01-004","CL-2016-01-001","207556/Z/17/Z","212516/Z/18/Z","MR/K017047/1","MR/S005579/1","NF-SI-0514-10109","213555/Z/18/Z","206328/Z/17/Z","AMS-SGCL11-Duncan","MR/S035842/1"],"pubmed_authors":["Allison J","Furlong A","Haniffa M","Stark H","Stephenson E","Pointon L","Dunmore BJ","Tuong ZK","Spegarova JS","Bach K","O'Brien C","Phelan I","Patterson C","Rossi S","Wilson NK","Barnes JL","Smith CM","Polwarth G","Kennet J","Martin J","Stefano GD","Horsfall D","Publico C","Calero-Nieto FJ","Domingo J","Huhn O","Hunter K","Coupland P","Bradley JR","Omarjee O","Josipovic M","Perera M","Walker N","Calder J","Ivers T","Sanchez-Gonzalez A","Epping M","Fawke S","Hambleton S","Dougan G","Kourampa J","Polanski K","De Bie EMDD","Mescia F","Clatworthy MR","Richoz N","Marioni JC","Laurenti E","Ansaripour A","Graf S","Prigmore E","Sutcliffe R","Clapham-Riley D","Summers C","Meyer KB","Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID-19 BioResource Collaboration","De Sa A","Matara C","McGrath J","van Dongen S","Jones E","de Wilton A","Barr AS","Coxhead JM","Meadows A","Lyons PA","Webb S","Kiselev VY","Rostron AJ","Bunclark K","Simpson AJ","Turner L","Selvan M","Morgan MD","Yoshida M","Muldoon F","Price J","Linger R","Schim van der Loeff ICD","Nikolic MZ","Engelbert J","Lehner PJ","Tong Z","Kingston N","Bacardit J","Sungnak W","Olabi B","Gray J","Jose S","Wood J","Wilson C","Nice F","Bergamaschi L","Baker S","Marsden J","Mackay M","Botting RA","Polgarova P","Bullman B","Hess C","Huang C","Rastall R","Lindeboom RGH","Ruffolo V","Huang N","Weekes MP","Papadia S","Hanrath AT","Saleh A","Hodgson J","Michael A","Saunders C","Smith KGC","Caputo D","Goh I","Dann E","Reynolds G","Gothe F","Treacy C","Elmer A","Mather MW","Wylot M","Jarvis I","Butcher H","Jardine L","Worlock KB","Tordesillas H","Tilly T","Sharma R","Webster J","Gopee N","Ribeiro C","Gupta RK","Townsend P","Janes SM","Spencer S","Owehand WH","Bower G","Kania K","King HW","Gardner LCS","Canna L","Dewhurst E","Gresley EL","Scott J","Okecha G","Pond N","Legchenko E","Betancourt A","Hewitt S","Rowlands J","Saigal A","Duncan CJA","Stirrups KE","Kumasaka N","Toshner M","Jackson S","Baker KF","Gottgens B","Thaventhiran JED","Goodfellow IG","Bucke A","Kasanicki M","Kilich E","Payne RP","Meloy S","Ovington N","O'Donnell C","Mende N","Shih J","Mwaura L","Fuller S","Matheson NJ","Harris J","Yong C","Teichmann SA","Graves B"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell multi-omics analysis of the immune response in COVID-19.","description":"Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts (CD16<sup>+</sup>C1QA/B/C<sup>+</sup>) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34<sup>+</sup> hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by ex","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-04-30T00:19:45.175Z","creation":"2025-02-19T03:43:17.998Z"},"accession":"S-EPMC8121667","cross_references":{"pubmed":["33879890"],"doi":["10.1038/s41591-021-01329-2"]}}