{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Choi Y"],"funding":["Rural Development Administration","National Research Foundation of Korea"],"pagination":["582"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8121941"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(1)"],"pubmed_abstract":["The conjugative plasmid (pBV71) possibly confers a selective advantage to Bacillus velezensis strain GH1-13, although a selective marker gene is yet to be identified. Here we show that few non-mucoid wild-type GH1-13 cells are spontaneously converted to mucoid variants with or without the loss of pBV71. Mucoid phenotypes, which contain or lack the plasmid, become sensitive to bacitracin, gramicidin, selenite, and tellurite. Using the differences in antibiotic resistance and phenotype, we isolated a reverse complement (COM) and a transconjugant of strain FZB42 with the native pBV71. Transformed COM and FZB42p cells were similar to the wild-type strain GH1-13 with high antibiotic resistance and slow growth rates on lactose compared to those of mucoid phenotypes. RT-PCR analysis revealed that"],"journal":["Communications biology"],"pubmed_title":["A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13."],"pmcid":["PMC8121941"],"funding_grant_id":["PJ01497402","PJ01246706","NRF-2018R1A5A1025077","NRF-2019R1F1A1062989"],"pubmed_authors":["Kim S","Nguyen MP","Tran LVH","Lee CW","Kim YH","Pham H","Choi Y","Kim J","Song J"],"additional_accession":[]},"is_claimable":false,"name":"A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13.","description":"The conjugative plasmid (pBV71) possibly confers a selective advantage to Bacillus velezensis strain GH1-13, although a selective marker gene is yet to be identified. Here we show that few non-mucoid wild-type GH1-13 cells are spontaneously converted to mucoid variants with or without the loss of pBV71. Mucoid phenotypes, which contain or lack the plasmid, become sensitive to bacitracin, gramicidin, selenite, and tellurite. Using the differences in antibiotic resistance and phenotype, we isolated a reverse complement (COM) and a transconjugant of strain FZB42 with the native pBV71. Transformed COM and FZB42p cells were similar to the wild-type strain GH1-13 with high antibiotic resistance and slow growth rates on lactose compared to those of mucoid phenotypes. RT-PCR analysis revealed that","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-08T10:36:21.841Z","creation":"2025-04-20T01:18:17.852Z"},"accession":"S-EPMC8121941","cross_references":{"pubmed":["33990691"],"doi":["10.1038/s42003-021-02107-z"]}}