biostudies-literatureEhinger YAix-Marseille UniversitéInstitut National de la Santé et de la Recherche MédicaleAFM-TéléthonAssociation Française du Syndrome de Rett (AFSR)Promex Stiftung Für Die ForschungRettsyndrome.orgCSRD VA4316https://www.ebi.ac.uk/biostudies/studies/S-EPMC8122273biostudies-literatureUnknown22(9)Mutations in the X-linked <i>MECP2</i> gene are responsible for Rett syndrome (RTT), a severe neurological disorder. MECP2 is a transcriptional modulator that finely regulates the expression of many genes, specifically in the central nervous system. Several studies have functionally linked the loss of MECP2 in astrocytes to the appearance and progression of the RTT phenotype in a non-cell autonomous manner and mechanisms are still unknown. Here, we used primary astroglial cells from <i>Mecp2</i>-deficient (KO) pups to identify deregulated secreted proteins. Using a differential quantitative proteomic analysis, twenty-nine proteins have been identified and four were confirmed by Western blotting with new samples as significantly deregulated. To further verify the functional relevance of theInternational journal of molecular sciencesAnalysis of Astroglial Secretomic Profile in the Mecp2-Deficient Male Mouse Model of Rett Syndrome.PMC812227312(Strategic pole MNH Decrypt)345Cunin VBourgoin-Voillard SSeve MBorloz ERoux JCBorges-Correia AMatagne VVillard LEhinger YfalseAnalysis of Astroglial Secretomic Profile in the Mecp2-Deficient Male Mouse Model of Rett Syndrome.Mutations in the X-linked <i>MECP2</i> gene are responsible for Rett syndrome (RTT), a severe neurological disorder. MECP2 is a transcriptional modulator that finely regulates the expression of many genes, specifically in the central nervous system. Several studies have functionally linked the loss of MECP2 in astrocytes to the appearance and progression of the RTT phenotype in a non-cell autonomous manner and mechanisms are still unknown. Here, we used primary astroglial cells from <i>Mecp2</i>-deficient (KO) pups to identify deregulated secreted proteins. Using a differential quantitative proteomic analysis, twenty-nine proteins have been identified and four were confirmed by Western blotting with new samples as significantly deregulated. To further verify the functional relevance of the2021-01-01T00:00:00Z2021 Apr2026-04-08T09:10:23.116Z2025-04-05T10:29:22.893ZS-EPMC81222733391925310.3390/ijms22094316