{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(1)"],"submitter":["Yang X"],"funding":["Canada Foundation for Innovation John R Evans Leaders Fund","Carcinoid and Neuroendocrine Tumor Society Canada","Southeastern Ontario Academic Medical Organization","Ontario Research Fund-Research Infrastructure","Academic Health Sciences Center Alternative Funding Plan Innovation Fund"],"pubmed_abstract":["Lung carcinoids are variably aggressive and mechanistically understudied neuroendocrine neoplasms (NENs). Here, we identified and elucidated the function of a miR-375/yes-associated protein (YAP) axis in lung carcinoid (H727) cells. miR-375 and YAP are respectively high and low expressed in wild-type H727 cells. Following lentiviral CRISPR/Cas9-mediated miR-375 depletion, we identified distinct transcriptomic changes including dramatic YAP upregulation. We also observed a significant decrease in neuroendocrine differentiation and substantial reductions in cell proliferation, transformation, and tumor growth in cell culture and xenograft mouse disease models. Similarly, YAP overexpression resulted in distinct and partially overlapping transcriptomic changes, phenocopying the effects of miR-"],"journal":["Scientific reports"],"pagination":["10455"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8129150"],"repository":["biostudies-literature"],"pubmed_title":["A miR-375/YAP axis regulates neuroendocrine differentiation and tumorigenesis in lung carcinoid cells."],"pmcid":["PMC8129150"],"pubmed_authors":["Yang X","Nanayakkara J","Xu M","Wong JJM","Renwick N","Hafner M","Saghafinia S","Nicol CJB","Claypool D","Wang X","Michael IP"],"additional_accession":[]},"is_claimable":false,"name":"A miR-375/YAP axis regulates neuroendocrine differentiation and tumorigenesis in lung carcinoid cells.","description":"Lung carcinoids are variably aggressive and mechanistically understudied neuroendocrine neoplasms (NENs). Here, we identified and elucidated the function of a miR-375/yes-associated protein (YAP) axis in lung carcinoid (H727) cells. miR-375 and YAP are respectively high and low expressed in wild-type H727 cells. Following lentiviral CRISPR/Cas9-mediated miR-375 depletion, we identified distinct transcriptomic changes including dramatic YAP upregulation. We also observed a significant decrease in neuroendocrine differentiation and substantial reductions in cell proliferation, transformation, and tumor growth in cell culture and xenograft mouse disease models. Similarly, YAP overexpression resulted in distinct and partially overlapping transcriptomic changes, phenocopying the effects of miR-","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-06-11T06:20:20.623Z","creation":"2025-04-04T11:57:50.857Z"},"accession":"S-EPMC8129150","cross_references":{"pubmed":["34001972"],"doi":["10.1038/s41598-021-89855-4"]}}