{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kurhade SE"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["12313-12318"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8131250"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["60(22)"],"pubmed_abstract":["An engineered cyanovirin-N homologue that exhibits specificity for high mannose N-glycans has been constructed to aid type I α 1,2-mannosidase inhibitor discovery and development. Engineering the lectins C-terminus permitted facile functionalization with fluorophores via a sortase and click strategy. The resulting lectin constructs exhibit specificity for cells presenting high mannose N-glycans. Importantly, these lectin constructs can also be applied to specifically assess changes in cell surface glycosylation induced by type I mannosidase inhibitors. Testing the utility of these lectin constructs led to the discovery of type I mannosidase inhibitors with nanomolar potency. Cumulatively, these findings reveal the specificity and utility of the functionalized cyanovirin-N homologue constru"],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["Functionalized High Mannose-Specific Lectins for the Discovery of Type I Mannosidase Inhibitors."],"pmcid":["PMC8131250"],"funding_grant_id":["P20 GM113117","GM113117","P20 GM103638","GM110761","P50 GM069663","P30 GM110761"],"pubmed_authors":["Gao FP","Weiner JD","Farrell MP","Kurhade SE"],"additional_accession":[]},"is_claimable":false,"name":"Functionalized High Mannose-Specific Lectins for the Discovery of Type I Mannosidase Inhibitors.","description":"An engineered cyanovirin-N homologue that exhibits specificity for high mannose N-glycans has been constructed to aid type I α 1,2-mannosidase inhibitor discovery and development. Engineering the lectins C-terminus permitted facile functionalization with fluorophores via a sortase and click strategy. The resulting lectin constructs exhibit specificity for cells presenting high mannose N-glycans. Importantly, these lectin constructs can also be applied to specifically assess changes in cell surface glycosylation induced by type I mannosidase inhibitors. Testing the utility of these lectin constructs led to the discovery of type I mannosidase inhibitors with nanomolar potency. Cumulatively, these findings reveal the specificity and utility of the functionalized cyanovirin-N homologue constru","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-31T09:48:52.071Z","creation":"2025-02-18T23:32:22.023Z"},"accession":"S-EPMC8131250","cross_references":{"pubmed":["33728787"],"doi":["10.1002/anie.202101249"]}}