{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tirgar P"],"funding":["National Institute for Genetic Engineering and Biotechnology"],"pagination":["034104"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8133792"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(3)"],"pubmed_abstract":["Embryo vitrification is a fundamental practice in assisted reproduction and fertility preservation. A key step of this process is replacing the internal water with cryoprotectants (CPAs) by transferring embryos from an isotonic to a hypertonic solution of CPAs. However, this applies an abrupt osmotic shock to embryos, resulting in molecular damages that have long been a source of concern. In this study, we introduce a standalone microfluidic system to automate the manual process and minimize the osmotic shock applied to embryos. This device provides the same final CPA concentrations as the manual method but with a gradual increase over time instead of sudden increases. Our system allows the introduction of the dehydrating non-permeating CPA, sucrose, from the onset of CPA-water exchange, w"],"journal":["Biomicrofluidics"],"pubmed_title":["Toward embryo cryopreservation-on-a-chip: A standalone microfluidic platform for gradual loading of cryoprotectants to minimize cryoinjuries."],"pmcid":["PMC8133792"],"funding_grant_id":["677"],"pubmed_authors":["Shoushtari Zadeh Naseri A","AzizMohseni S","Ehrlicher A","Zandi G","Tirgar P","Ziaie N","Najafi M","Kazemi P","Fayazi S","Dashtizad M","Sarmadi F"],"additional_accession":[]},"is_claimable":false,"name":"Toward embryo cryopreservation-on-a-chip: A standalone microfluidic platform for gradual loading of cryoprotectants to minimize cryoinjuries.","description":"Embryo vitrification is a fundamental practice in assisted reproduction and fertility preservation. A key step of this process is replacing the internal water with cryoprotectants (CPAs) by transferring embryos from an isotonic to a hypertonic solution of CPAs. However, this applies an abrupt osmotic shock to embryos, resulting in molecular damages that have long been a source of concern. In this study, we introduce a standalone microfluidic system to automate the manual process and minimize the osmotic shock applied to embryos. This device provides the same final CPA concentrations as the manual method but with a gradual increase over time instead of sudden increases. Our system allows the introduction of the dehydrating non-permeating CPA, sucrose, from the onset of CPA-water exchange, w","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-07-09T11:23:04.612Z","creation":"2026-07-09T10:46:47.582Z"},"accession":"S-EPMC8133792","cross_references":{"pubmed":["34025896"],"doi":["10.1063/5.0047185"]}}