{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yokoyama R"],"funding":["Japanese Society for the Promotion of Science (JSPS) and the Uehara Memorial Foundation","National Science Foundation"],"pagination":["671-696"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8136874"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(3)"],"pubmed_abstract":["The plant shikimate pathway directs bulk carbon flow toward biosynthesis of aromatic amino acids (AAAs, i.e. tyrosine, phenylalanine, and tryptophan) and numerous aromatic phytochemicals. The microbial shikimate pathway is feedback inhibited by AAAs at the first enzyme, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DHS). However, AAAs generally do not inhibit DHS activities from plant extracts and how plants regulate the shikimate pathway remains elusive. Here, we characterized recombinant Arabidopsis thaliana DHSs (AthDHSs) and found that tyrosine and tryptophan inhibit AthDHS2, but not AthDHS1 or AthDHS3. Mixing AthDHS2 with AthDHS1 or 3 attenuated its inhibition. The AAA and phenylpropanoid pathway intermediates chorismate and caffeate, respectively, strongly inhibited all AthDH"],"journal":["The Plant cell"],"pubmed_title":["The entry reaction of the plant shikimate pathway is subjected to highly complex metabolite-mediated regulation."],"pmcid":["PMC8136874"],"funding_grant_id":["MCB-1818040","2017-2444","201730074"],"pubmed_authors":["Yokoyama R","Maeda HA","de Oliveira MVV","Kleven B"],"additional_accession":[]},"is_claimable":false,"name":"The entry reaction of the plant shikimate pathway is subjected to highly complex metabolite-mediated regulation.","description":"The plant shikimate pathway directs bulk carbon flow toward biosynthesis of aromatic amino acids (AAAs, i.e. tyrosine, phenylalanine, and tryptophan) and numerous aromatic phytochemicals. The microbial shikimate pathway is feedback inhibited by AAAs at the first enzyme, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DHS). However, AAAs generally do not inhibit DHS activities from plant extracts and how plants regulate the shikimate pathway remains elusive. Here, we characterized recombinant Arabidopsis thaliana DHSs (AthDHSs) and found that tyrosine and tryptophan inhibit AthDHS2, but not AthDHS1 or AthDHS3. Mixing AthDHS2 with AthDHS1 or 3 attenuated its inhibition. The AAA and phenylpropanoid pathway intermediates chorismate and caffeate, respectively, strongly inhibited all AthDH","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-07-09T11:49:18.685Z","creation":"2022-02-11T13:01:24.484Z"},"accession":"S-EPMC8136874","cross_references":{"pubmed":["33955484"],"doi":["10.1093/plcell/koaa042"]}}