biostudies-literatureLiu CInnovative Research Group Project of the National Natural Science Foundation of Chinathe Strategic Priority Research Program of Chinese Academy of Sciences119https://www.ebi.ac.uk/biostudies/studies/S-EPMC8140505biostudies-literatureUnknown9(1)<h4>Background</h4>In gut microbiome studies, the cultured gut microbial resource plays essential roles, such as helping to unravel gut microbial functions and host-microbe interactions. Although several major studies have been performed to elucidate the cultured human gut microbiota, up to 70% of the Unified Human Gastrointestinal Genome species have not been cultured to date. Large-scale gut microbial isolation and identification as well as availability to the public are imperative for gut microbial studies and further characterizing human gut microbial functions.<h4>Results</h4>In this study, we constructed a human Gut Microbial Biobank (hGMB; homepage: hgmb.nmdc.cn ) through the cultivation of 10,558 isolates from 31 sample mixtures of 239 fresh fecal samples from healthy Chinese volunMicrobiomeEnlightening the taxonomy darkness of human gut microbiomes with a cultured biobank.PMC8140505XDB380203002019YFA0905601Shi WYZhou NWu LDu MXChen HHZhou YHan SSYu HYWang YJLiu SJJiang CYXin YHLi DHNiu PXJiang MZMa JLiu HWLiu CAbuduaini RfalseEnlightening the taxonomy darkness of human gut microbiomes with a cultured biobank.<h4>Background</h4>In gut microbiome studies, the cultured gut microbial resource plays essential roles, such as helping to unravel gut microbial functions and host-microbe interactions. Although several major studies have been performed to elucidate the cultured human gut microbiota, up to 70% of the Unified Human Gastrointestinal Genome species have not been cultured to date. Large-scale gut microbial isolation and identification as well as availability to the public are imperative for gut microbial studies and further characterizing human gut microbial functions.<h4>Results</h4>In this study, we constructed a human Gut Microbial Biobank (hGMB; homepage: hgmb.nmdc.cn ) through the cultivation of 10,558 isolates from 31 sample mixtures of 239 fresh fecal samples from healthy Chinese volun2021-01-01T00:00:00Z2021 May2026-04-12T15:45:21.239Z2022-02-10T15:20:21.373ZS-EPMC81405053402071410.1186/s40168-021-01064-3