biostudies-literatureSubiros-Funosas RCancer Research UKEuropean Research CouncilMinisterio de Economía y CompetitividadWellcome TrustDepartament d'Innovació, Universitats i Empresa, Generalitat de CatalunyaEngineering and Physical Sciences Research Council1368-1374https://www.ebi.ac.uk/biostudies/studies/S-EPMC8148049biostudies-literatureUnknown11(5)Keratin 1 (KRT1) is overexpressed in squamous carcinomas and associated with aggressive pathologies in breast cancer. Herein we report the design and preparation of the first Trp-based red fluorogenic amino acid, which is synthetically accessible in a few steps and displays excellent photophysical properties, and its application in a minimally-disruptive labelling strategy to prepare a new fluorogenic cyclopeptide for imaging of KRT1+ cells in whole intact tumour tissues.Chemical scienceFluorogenic Trp(redBODIPY) cyclopeptide targeting keratin 1 for imaging of aggressive carcinomas.PMC8148049C49791/A17367PIII-006173672017 SGR 1439ERA-NET PCIN-2015-224771443EP/M507258/1CTQ2015-67870-P7163791783744Sintes MMa RVendrell MZhang CBGhashghaei OPappalardo MBarth NDHo VCLBarril XSubiros-Funosas RLavilla RQian BZMendive-Tapia LfalseFluorogenic Trp(redBODIPY) cyclopeptide targeting keratin 1 for imaging of aggressive carcinomas.Keratin 1 (KRT1) is overexpressed in squamous carcinomas and associated with aggressive pathologies in breast cancer. Herein we report the design and preparation of the first Trp-based red fluorogenic amino acid, which is synthetically accessible in a few steps and displays excellent photophysical properties, and its application in a minimally-disruptive labelling strategy to prepare a new fluorogenic cyclopeptide for imaging of KRT1+ cells in whole intact tumour tissues.2019-01-01T00:00:00Z2019 Dec2026-05-08T14:18:17.815Z2022-02-10T14:38:09.35ZS-EPMC81480493412326110.1039/c9sc05558d