{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hutchinson-Bunch C"],"funding":["NIDDK NIH HHS","National Cancer Institute"],"pagination":["12660-12666"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8154127"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(19)"],"pubmed_abstract":["Isobaric labeling via tandem mass tag (TMT) reagents enables sample multiplexing prior to LC-MS/MS, facilitating high-throughput large-scale quantitative proteomics. Consistent and efficient labeling reactions are essential to achieve robust quantification; therefore, embedded in our clinical proteomic protocol is a quality control (QC) sample that contains a small aliquot from each sample within a TMT set, referred to as \"Mixing QC.\" This Mixing QC enables the detection of TMT labeling issues by LC-MS/MS before combining the full samples to allow for salvaging of poor TMT labeling reactions. While TMT labeling is a valuable tool, factors leading to poor reactions are not fully studied. We observed that relabeling does not necessarily rescue TMT reactions and that peptide samples sometimes"],"journal":["ACS omega"],"pubmed_title":["Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH."],"pmcid":["PMC8154127"],"funding_grant_id":["R01 DK122160","U01 CA214116","U24 DK112349","U01 CA227544"],"pubmed_authors":["Qian WJ","Sanford JA","Piehowski PD","Hansen JR","Gritsenko MA","Rodland KD","Hutchinson-Bunch C","Adkins JN"],"additional_accession":[]},"is_claimable":false,"name":"Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH.","description":"Isobaric labeling via tandem mass tag (TMT) reagents enables sample multiplexing prior to LC-MS/MS, facilitating high-throughput large-scale quantitative proteomics. Consistent and efficient labeling reactions are essential to achieve robust quantification; therefore, embedded in our clinical proteomic protocol is a quality control (QC) sample that contains a small aliquot from each sample within a TMT set, referred to as \"Mixing QC.\" This Mixing QC enables the detection of TMT labeling issues by LC-MS/MS before combining the full samples to allow for salvaging of poor TMT labeling reactions. While TMT labeling is a valuable tool, factors leading to poor reactions are not fully studied. We observed that relabeling does not necessarily rescue TMT reactions and that peptide samples sometimes","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2025-04-18T16:05:26.885Z","creation":"2022-02-10T12:57:08.392Z"},"accession":"S-EPMC8154127","cross_references":{"pubmed":["34056417"],"doi":["10.1021/acsomega.1c00776"]}}