biostudies-literaturePlantz MANational Institute of Arthritis and Musculoskeletal and Skin DiseasesU.S. Department of EnergyArgonne National LaboratoryOffice of ScienceNational Institutes of HealthNIAMS NIH HHS146-158https://www.ebi.ac.uk/biostudies/studies/S-EPMC8154748biostudies-literatureUnknown127We recently developed a recombinant growth factor-free bone regenerative scaffold composed of stoichiometric hydroxyapatite (HA) ceramic particles and human demineralized bone matrix (DBM) particles (HA-DBM). Here, we performed the first pre-clinical comparative evaluation of HA-DBM relative to the industry standard and established positive control, recombinant human bone morphogenetic protein-2 (rhBMP-2), using a rat posterolateral spinal fusion model (PLF). Female Sprague-Dawley rats underwent bilateral L4-L5 PLF with implantation of the HA-DBM scaffold or rhBMP-2. Fusion was evaluated using radiography and blinded manual palpation, while biomechanical testing quantified the segmental flexion-extension range-of-motion (ROM) and stiffness of the fused segments at 8-weeks postoperatively. Acta biomaterialiaOsteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model.PMC8154748R01 AR069580DE-AC02-06CH11357R01AR069580Blank KRMinardi SShah RNHallman MPlantz MAYun CStock SRGreene ACHsu WKLyons JGMuriuki MJeong SYamaguchi JTHavey RMEllenbogen DJPatwardhan AGHsu ELJakus AEfalseOsteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model.We recently developed a recombinant growth factor-free bone regenerative scaffold composed of stoichiometric hydroxyapatite (HA) ceramic particles and human demineralized bone matrix (DBM) particles (HA-DBM). Here, we performed the first pre-clinical comparative evaluation of HA-DBM relative to the industry standard and established positive control, recombinant human bone morphogenetic protein-2 (rhBMP-2), using a rat posterolateral spinal fusion model (PLF). Female Sprague-Dawley rats underwent bilateral L4-L5 PLF with implantation of the HA-DBM scaffold or rhBMP-2. Fusion was evaluated using radiography and blinded manual palpation, while biomechanical testing quantified the segmental flexion-extension range-of-motion (ROM) and stiffness of the fused segments at 8-weeks postoperatively. 2021-01-01T00:00:00Z2021 Jun2025-04-04T20:15:35.465Z2025-04-04T20:15:35.465ZS-EPMC81547483383157610.1016/j.actbio.2021.03.060