{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Biagioni A"],"funding":["Associazione Italiana per la Ricerca sul Cancro","Ente Cassa di Risparmio di Firenze"],"pubmed_abstract":["uPAR is a globular protein, tethered to the cell membrane by a GPI-anchor involved in several cancer-related properties and its overexpression commonly correlates with poor prognosis and metastasis. We investigated the consequences of uPAR irreversible loss in human melanoma and colon cancer cell lines, knocking out its expression by CRISPR/Cas9. We analyzed through flow cytometry, western blotting and qPCR, the modulation of the most known cancer stem cells-associated genes and the EGFR while we observed the proliferation rate exploiting 2D and 3D cellular models. We also generated uPAR \"rescue\" expression cell lines as well as we promoted the expression of only its 3'UTR to demonstrate the involvement of uPAR mRNA in tumor progression. Knocking out PLAUR, uPAR-encoding gene, we observed "],"journal":["Frontiers in oncology"],"pagination":["663225"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8163229"],"repository":["biostudies-literature"],"pubmed_title":["CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines."],"pmcid":["PMC8163229"],"pubmed_authors":["Calorini L","Li Santi A","Margheri F","Scavone F","Ragno P","Bianchini F","Peppicelli S","Chilla A","Fibbi G","Del Rosso M","Laurenzana A","Andreucci E","Biagioni A"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines.","description":"uPAR is a globular protein, tethered to the cell membrane by a GPI-anchor involved in several cancer-related properties and its overexpression commonly correlates with poor prognosis and metastasis. We investigated the consequences of uPAR irreversible loss in human melanoma and colon cancer cell lines, knocking out its expression by CRISPR/Cas9. We analyzed through flow cytometry, western blotting and qPCR, the modulation of the most known cancer stem cells-associated genes and the EGFR while we observed the proliferation rate exploiting 2D and 3D cellular models. We also generated uPAR \"rescue\" expression cell lines as well as we promoted the expression of only its 3'UTR to demonstrate the involvement of uPAR mRNA in tumor progression. Knocking out PLAUR, uPAR-encoding gene, we observed ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-19T16:36:11.744Z","creation":"2022-02-10T12:41:00.509Z"},"accession":"S-EPMC8163229","cross_references":{"pubmed":["34055629"],"doi":["10.3389/fonc.2021.663225"]}}