biostudies-literaturePanczyk EMNational Institutes of HealthNIGMS NIH HHSNIH HHS5513-5520https://www.ebi.ac.uk/biostudies/studies/S-EPMC8168055biostudies-literatureUnknown93(13)Native mass spectrometry (nMS), particularly in conjunction with gas-phase ion mobility spectrometry measurements, has proven useful as a structural biology tool for evaluating the stoichiometry, conformation, and topology of protein complexes. Here, we demonstrate the combination of trapped ion mobility spectrometry (TIMS) and surface-induced dissociation (SID) on a Bruker SolariX XR 15 T FT-ICR mass spectrometer for the structural analysis of protein complexes. We successfully performed SID on mobility-selected protein complexes, including the streptavidin tetramer and cholera toxin B with bound ligands. Additionally, TIMS-SID was employed on a mixture of the peptides desArg1 and desArg9 bradykinin to mobility-separate and identify the individual peptides. Importantly, results show that Analytical chemistrySurface-Induced Dissociation of Protein Complexes Selected by Trapped Ion Mobility Spectrometry.PMC8168055S10 OD018507P41GM128577P41 GM128577Wysocki VHSnyder DTPanczyk EMPark MARidgeway MESomogyi AfalseSurface-Induced Dissociation of Protein Complexes Selected by Trapped Ion Mobility Spectrometry.Native mass spectrometry (nMS), particularly in conjunction with gas-phase ion mobility spectrometry measurements, has proven useful as a structural biology tool for evaluating the stoichiometry, conformation, and topology of protein complexes. Here, we demonstrate the combination of trapped ion mobility spectrometry (TIMS) and surface-induced dissociation (SID) on a Bruker SolariX XR 15 T FT-ICR mass spectrometer for the structural analysis of protein complexes. We successfully performed SID on mobility-selected protein complexes, including the streptavidin tetramer and cholera toxin B with bound ligands. Additionally, TIMS-SID was employed on a mixture of the peptides desArg1 and desArg9 bradykinin to mobility-separate and identify the individual peptides. Importantly, results show that 2021-01-01T00:00:00Z2021 Apr2025-04-05T14:05:38.422Z2025-04-05T14:05:38.422ZS-EPMC81680553375188710.1021/acs.analchem.0c05373