biostudies-literatureHe YKey Research and Development Projects of Shaanxi ProvinceNational Natural Science Foundation of China648277https://www.ebi.ac.uk/biostudies/studies/S-EPMC8170035biostudies-literatureUnknown12<i>Amygdalus pedunculata</i> Pall [Rosaceae, Prunus, <i>Prunus pedunculata</i> (Pall.) Maxim.] belongs to the Rosaceae family and is resistant to cold and drought. Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry and metabolomics were used to track the changes in bioactive metabolites during several stages of <i>Amygdalus pedunculata Pall</i> growth. A total of 827 different metabolites were detected, including 169 flavonoids, 68 organic acids, 35 terpenoids and 2 tannins. Flavonoid biosynthesis and flavone and flavonol biosynthesis were the main synthetic sources of flavonoids. Quercetin, isoquercitrin, and epicatechin as biomarkers related to growth and development were found. Quercetin connects the biosynthesis of flavonoids and the biosynthesis of flaFrontiers in plant scienceMetabolomic and Confocal Laser Scanning Microscopy (CLSM) Analyses Reveal the Important Function of Flavonoids in <i>Amygdalus pedunculata</i> Pall Leaves With Temporal Changes.PMC81700352019TSLNY03-022018ZDXM-NY-087He YLi CWang WPan LYang TShen YChen BfalseMetabolomic and Confocal Laser Scanning Microscopy (CLSM) Analyses Reveal the Important Function of Flavonoids in <i>Amygdalus pedunculata</i> Pall Leaves With Temporal Changes.<i>Amygdalus pedunculata</i> Pall [Rosaceae, Prunus, <i>Prunus pedunculata</i> (Pall.) Maxim.] belongs to the Rosaceae family and is resistant to cold and drought. Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry and metabolomics were used to track the changes in bioactive metabolites during several stages of <i>Amygdalus pedunculata Pall</i> growth. A total of 827 different metabolites were detected, including 169 flavonoids, 68 organic acids, 35 terpenoids and 2 tannins. Flavonoid biosynthesis and flavone and flavonol biosynthesis were the main synthetic sources of flavonoids. Quercetin, isoquercitrin, and epicatechin as biomarkers related to growth and development were found. Quercetin connects the biosynthesis of flavonoids and the biosynthesis of fla2021-01-01T00:00:00Z20212025-04-19T17:44:21.01Z2022-02-10T13:52:40.511ZS-EPMC81700353409361110.3389/fpls.2021.648277