{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vadivel K"],"funding":["Austrian Science Fund FWF","NHLBI NIH HHS","National Heart, Lung, and Blood Institute"],"pagination":["809-819"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8171065"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["77(Pt 6)"],"pubmed_abstract":["Coagulation factor VIIa (FVIIa) consists of a γ-carboxyglutamic acid (GLA) domain, two epidermal growth factor-like (EGF) domains and a protease domain. FVIIa binds three Mg<sup>2+</sup> ions and four Ca<sup>2+</sup> ions in the GLA domain, one Ca<sup>2+</sup> ion in the EGF1 domain and one Ca<sup>2+</sup> ion in the protease domain. Further, FVIIa contains an Na<sup>+</sup> site in the protease domain. Since Na<sup>+</sup> and water share the same number of electrons, Na<sup>+</sup> sites in proteins are difficult to distinguish from waters in X-ray structures. Here, to verify the Na<sup>+</sup> site in FVIIa, the structure of the FVIIa-soluble tissue factor (TF) complex was solved at 1.8 Å resolution containing Mg<sup>2+</sup>, Ca<sup>2+</sup> and Rb<sup>+</sup> ions. In this structure, "],"journal":["Acta crystallographica. Section D, Structural biology"],"pubmed_title":["Structure of human factor VIIa-soluble tissue factor with calcium, magnesium and rubidium."],"pmcid":["PMC8171065"],"funding_grant_id":["R01 HL141850","R01HL141850","P01 HL139420","P01HL139420","W1213"],"pubmed_authors":["Vadivel K","Krishnaswamy S","Padmanabhan K","Cascio D","Brandstetter H","Bajaj SP","Schmidt AE"],"additional_accession":[]},"is_claimable":false,"name":"Structure of human factor VIIa-soluble tissue factor with calcium, magnesium and rubidium.","description":"Coagulation factor VIIa (FVIIa) consists of a γ-carboxyglutamic acid (GLA) domain, two epidermal growth factor-like (EGF) domains and a protease domain. FVIIa binds three Mg<sup>2+</sup> ions and four Ca<sup>2+</sup> ions in the GLA domain, one Ca<sup>2+</sup> ion in the EGF1 domain and one Ca<sup>2+</sup> ion in the protease domain. Further, FVIIa contains an Na<sup>+</sup> site in the protease domain. Since Na<sup>+</sup> and water share the same number of electrons, Na<sup>+</sup> sites in proteins are difficult to distinguish from waters in X-ray structures. Here, to verify the Na<sup>+</sup> site in FVIIa, the structure of the FVIIa-soluble tissue factor (TF) complex was solved at 1.8 Å resolution containing Mg<sup>2+</sup>, Ca<sup>2+</sup> and Rb<sup>+</sup> ions. In this structure, ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-25T16:52:29.023Z","creation":"2025-02-18T23:32:14.746Z"},"accession":"S-EPMC8171065","cross_references":{"pubmed":["34076594"],"doi":["10.1107/S2059798321003922","10.1107/s2059798321003922"]}}