{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(6)"],"submitter":["Huang Y"],"funding":["AstraZeneca"],"pubmed_abstract":["Quantification of endogenous biomarkers in clinical studies requires careful evaluation of a number of assay performance parameters. Comparisons of absolute values from several clinical studies can enable retrospective analyses further elucidating the biology of a given biomarker across various study populations. We characterized the performance of a highly multiplex bioanalytical method for quantification of phosphatidylinositols (PI). Hydrophilic interaction chromatography (HILIC) and multiple reaction monitoring (MRM) were employed for targeted multiplex quantification. Odd-chain PI species that are not normally present in human plasma were utilized as surrogate analytes (SA) to assess various assay performance parameters and establish a definitive dynamic linear range for PI lipids. To"],"journal":["PloS one"],"pagination":["e0252426"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8177664"],"repository":["biostudies-literature"],"pubmed_title":["Differences in levels of phosphatidylinositols in healthy and stable Coronary Artery Disease subjects revealed by HILIC-MRM method with SERRF normalization."],"pmcid":["PMC8177664"],"pubmed_authors":["Huang Y","Rosenbaum AI","Grimsby JS","Liang M","Wen D","Mu R"],"additional_accession":[]},"is_claimable":false,"name":"Differences in levels of phosphatidylinositols in healthy and stable Coronary Artery Disease subjects revealed by HILIC-MRM method with SERRF normalization.","description":"Quantification of endogenous biomarkers in clinical studies requires careful evaluation of a number of assay performance parameters. Comparisons of absolute values from several clinical studies can enable retrospective analyses further elucidating the biology of a given biomarker across various study populations. We characterized the performance of a highly multiplex bioanalytical method for quantification of phosphatidylinositols (PI). Hydrophilic interaction chromatography (HILIC) and multiple reaction monitoring (MRM) were employed for targeted multiplex quantification. Odd-chain PI species that are not normally present in human plasma were utilized as surrogate analytes (SA) to assess various assay performance parameters and establish a definitive dynamic linear range for PI lipids. To","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-09T07:58:49.516Z","creation":"2022-02-10T14:07:43.965Z"},"accession":"S-EPMC8177664","cross_references":{"pubmed":["34086718"],"doi":["10.1371/journal.pone.0252426"]}}