{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["29(6)"],"submitter":["Li M"],"funding":["Foundation for Innovative Research Groups of the National Natural Science Foundation of China"],"pubmed_abstract":["Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated 9 (Cas9)-mediated loss-of-function screens are powerful tools for identifying genes responsible for diverse phenotypes. Here, we perturbed genes in melanoma cells to screen for genes involved in tumor escape from T cell-mediated killing. Multiple interferon gamma (IFNγ) signaling-related genes were enriched in melanoma cells resistant to T cell killing. In addition, deletion of the deubiquitinating protease ubiquitin specific peptidase 22 (USP22) in mouse melanoma (B16-OVA) cells decreased the efficacy of T cell-mediated killing, both in vitro and in vivo, while overexpression enhanced tumor-cell sensitivity to T (OT-I) cell-mediated killing. USP22 deficiency in both mouse and human melanoma ce"],"journal":["Molecular therapy : the journal of the American Society of Gene Therapy"],"pagination":["2108-2120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8178440"],"repository":["biostudies-literature"],"pubmed_title":["USP22 deficiency in melanoma mediates resistance to T cells through IFNγ-JAK1-STAT1 signal axis."],"pmcid":["PMC8178440"],"pubmed_authors":["Yang X","Han P","Qi X","Liang J","Li F","Gao Y","Lin H","Li M","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"USP22 deficiency in melanoma mediates resistance to T cells through IFNγ-JAK1-STAT1 signal axis.","description":"Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated 9 (Cas9)-mediated loss-of-function screens are powerful tools for identifying genes responsible for diverse phenotypes. Here, we perturbed genes in melanoma cells to screen for genes involved in tumor escape from T cell-mediated killing. Multiple interferon gamma (IFNγ) signaling-related genes were enriched in melanoma cells resistant to T cell killing. In addition, deletion of the deubiquitinating protease ubiquitin specific peptidase 22 (USP22) in mouse melanoma (B16-OVA) cells decreased the efficacy of T cell-mediated killing, both in vitro and in vivo, while overexpression enhanced tumor-cell sensitivity to T (OT-I) cell-mediated killing. USP22 deficiency in both mouse and human melanoma ce","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-19T18:17:56.148Z","creation":"2025-04-19T18:17:56.148Z"},"accession":"S-EPMC8178440","cross_references":{"pubmed":["33601053"],"doi":["10.1016/j.ymthe.2021.02.018"]}}