{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Habel N"],"funding":["Ligue Contre le Cancer","Fondation ARC pour la Recherche sur le Cancer (ARC Foundation for Cancer Research)","Agence Nationale de la Recherche (French National Research Agency)","Institut National Du Cancer (French National Cancer Institute)"],"pagination":["1837-1848"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8184796"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(6)"],"pubmed_abstract":["Ubiquitination by serving as a major degradation signal of proteins, but also by controlling protein functioning and localization, plays critical roles in most key cellular processes. Here, we show that MITF, the master transcription factor in melanocytes, controls ubiquitination in melanoma cells. We identified FBXO32, a component of the SCF E3 ligase complex as a new MITF target gene. FBXO32 favors melanoma cell migration, proliferation, and tumor development in vivo. Transcriptomic analysis shows that FBXO32 knockdown induces a global change in melanoma gene expression profile. These include the inhibition of CDK6 in agreement with an inhibition of cell proliferation and invasion upon FBXO32 silencing. Furthermore, proteomic analysis identifies SMARC4, a component of the chromatin remod"],"journal":["Cell death and differentiation"],"pubmed_title":["FBXO32 links ubiquitination to epigenetic reprograming of melanoma cells."],"pmcid":["PMC8184796"],"funding_grant_id":["équipe labellisée 2019 to RB","INCa-10573","Post doc fellowship","AAPEAC2019.LCC/STD","Labex Signalife PhD Program_ANR-11 LABEX-0028-01","équipe labellisée 2019"],"pubmed_authors":["Bertolotto C","Nguyen S","Habel N","Gay AS","El-Hachem N","Ballotti R","Nottet N","Giuliano S","Paillerets BB","Hadhiri-Bzioueche H","Horak P","Debayle D","Beranger G","Soysouvanh F"],"additional_accession":[]},"is_claimable":false,"name":"FBXO32 links ubiquitination to epigenetic reprograming of melanoma cells.","description":"Ubiquitination by serving as a major degradation signal of proteins, but also by controlling protein functioning and localization, plays critical roles in most key cellular processes. Here, we show that MITF, the master transcription factor in melanocytes, controls ubiquitination in melanoma cells. We identified FBXO32, a component of the SCF E3 ligase complex as a new MITF target gene. FBXO32 favors melanoma cell migration, proliferation, and tumor development in vivo. Transcriptomic analysis shows that FBXO32 knockdown induces a global change in melanoma gene expression profile. These include the inhibition of CDK6 in agreement with an inhibition of cell proliferation and invasion upon FBXO32 silencing. Furthermore, proteomic analysis identifies SMARC4, a component of the chromatin remod","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2026-05-07T23:04:51.645Z","creation":"2022-02-10T14:09:57.368Z"},"accession":"S-EPMC8184796","cross_references":{"pubmed":["33462405"],"doi":["10.1038/s41418-020-00710-x"]}}