biostudies-literatureChavez JDNCRR NIH HHSNIAID NIH HHSNHLBI NIH HHSNIGMS NIH HHS248-261https://www.ebi.ac.uk/biostudies/studies/S-EPMC8184889biostudies-literatureUnknown41(2)The set of all intra- and intermolecular interactions, collectively known as the interactome, is currently an unmet challenge for any analytical method, but if measured, could provide unparalleled insight on molecular function in living systems. Developments and applications of chemical cross-linking and high-performance mass spectrometry technologies are beginning to reveal details on how proteins interact in cells and how protein conformations and interactions inside cells change with phenotype or during drug treatment or other perturbations. A major contributor to these advances is Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) technology and its implementation with accurate mass measurements on cross-linked peptide-pair precursor and fragment ions to enable impMass spectrometry reviewsApplications and advancements of FT-ICR-MS for interactome studies.PMC8184889S10 RR025107R35 GM136255U19 AI107775R01 HL110879R01 GM086688R01 RR023334R01 GM097112R01 HL144778Bruce JEChavez JDPark SGMohr JPfalseApplications and advancements of FT-ICR-MS for interactome studies.The set of all intra- and intermolecular interactions, collectively known as the interactome, is currently an unmet challenge for any analytical method, but if measured, could provide unparalleled insight on molecular function in living systems. Developments and applications of chemical cross-linking and high-performance mass spectrometry technologies are beginning to reveal details on how proteins interact in cells and how protein conformations and interactions inside cells change with phenotype or during drug treatment or other perturbations. A major contributor to these advances is Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) technology and its implementation with accurate mass measurements on cross-linked peptide-pair precursor and fragment ions to enable imp2022-01-01T00:00:00Z2022 Mar2025-04-04T08:53:10.638Z2025-04-04T08:53:10.638ZS-EPMC81848893328994010.1002/mas.21675