biostudies-literatureYuan XNational Basic Research Program of ChinaNational Basic Research Program of China (973 Program)National Natural Science Foundation of ChinaNational Nature Science Foundation of China107https://www.ebi.ac.uk/biostudies/studies/S-EPMC8186080biostudies-literatureUnknown11(1)<h4>Background</h4>Autophagy is required for oogenesis and plays a critical role in response to aging caused by oxidative stress. However, there have been no reports on regulation of cytoprotective autophagy in female germline stem cells (FGSCs) in response to aging caused by oxidative stress.<h4>Results</h4>We found that Spermidine (SPD) significantly increased protein expression of autophagy markers microtubule-associated protein 1 light chain 3 beta-II (MAP1LC3B-II/LC3B-II) and sequestosome-1/p62 (SQSTM1/p62), and evoked autophagic flux in FGSCs. Moreover, SPD increased the number and viability of FGSCs in vitro. Further, we found that SPD significantly reduced basal or hydrogen peroxide (H₂O₂)-induced up-regulated protein expression of the aging markers, cyclin deCell & bioscienceSpermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression.PMC8186080817201080172017YFA0504201618278142018YFC1003501Wu JHu XPei XYuan XTian GGfalseSpermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression.<h4>Background</h4>Autophagy is required for oogenesis and plays a critical role in response to aging caused by oxidative stress. However, there have been no reports on regulation of cytoprotective autophagy in female germline stem cells (FGSCs) in response to aging caused by oxidative stress.<h4>Results</h4>We found that Spermidine (SPD) significantly increased protein expression of autophagy markers microtubule-associated protein 1 light chain 3 beta-II (MAP1LC3B-II/LC3B-II) and sequestosome-1/p62 (SQSTM1/p62), and evoked autophagic flux in FGSCs. Moreover, SPD increased the number and viability of FGSCs in vitro. Further, we found that SPD significantly reduced basal or hydrogen peroxide (H₂O₂)-induced up-regulated protein expression of the aging markers, cyclin de2021-01-01T00:00:00Z2021 Jun2026-05-09T05:06:04.46Z2022-02-10T14:15:45.705ZS-EPMC81860803409904110.1186/s13578-021-00614-4