{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Luo Y"],"funding":["National Natural Science Foundation of China-Guangdong Joint Fund"],"pagination":["659111"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8186282"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["Functional microorganisms play a vital role in removing environmental pollutants because of their diverse metabolic capability. Herein, a function-oriented fluorescence resonance energy transfer (FRET)-based graphene quantum dots (GQDs-M) probe was developed for the specific identification and accurate sorting of azo-degrading functional bacteria in the original location of environmental samples for large-scale culturing. First, nitrogen-doped GQDs (GQDs-N) were synthesized using a bottom-up strategy. Then, a GQDs-M probe was synthesized based on bonding FRET-based GQDs-N to an azo dye, methyl red, and the quenched fluorescence was recovered upon cleavage of the azo bond. Bioimaging confirmed the specific recognition capability of GQDs-M upon incubation with the target bacteria or environm"],"journal":["Frontiers in microbiology"],"pubmed_title":["Function-Oriented Graphene Quantum Dots Probe for Single Cell <i>in situ</i> Sorting of Active Microorganisms in Environmental Samples."],"pmcid":["PMC8186282"],"funding_grant_id":["91851202","U1701243"],"pubmed_authors":["Luo Q","Yang Y","Xu M","Mei C","Liao B","Luo Y","Liu F","Song J"],"additional_accession":[]},"is_claimable":false,"name":"Function-Oriented Graphene Quantum Dots Probe for Single Cell <i>in situ</i> Sorting of Active Microorganisms in Environmental Samples.","description":"Functional microorganisms play a vital role in removing environmental pollutants because of their diverse metabolic capability. Herein, a function-oriented fluorescence resonance energy transfer (FRET)-based graphene quantum dots (GQDs-M) probe was developed for the specific identification and accurate sorting of azo-degrading functional bacteria in the original location of environmental samples for large-scale culturing. First, nitrogen-doped GQDs (GQDs-N) were synthesized using a bottom-up strategy. Then, a GQDs-M probe was synthesized based on bonding FRET-based GQDs-N to an azo dye, methyl red, and the quenched fluorescence was recovered upon cleavage of the azo bond. Bioimaging confirmed the specific recognition capability of GQDs-M upon incubation with the target bacteria or environm","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-06-01T03:55:43.614Z","creation":"2022-02-10T14:23:40.301Z"},"accession":"S-EPMC8186282","cross_references":{"pubmed":["34113325"],"doi":["10.3389/fmicb.2021.659111"]}}