biostudies-literatureO'Connor SANational Institute of Neurological Disorders and StrokePew Charitable TrustsNational Cancer InstituteNCI NIH HHSNINDS NIH HHSArmed Forces Research Institute of Medical Sciencese9522https://www.ebi.ac.uk/biostudies/studies/S-EPMC8186478biostudies-literatureUnknown17(6)Single-cell RNA sequencing has emerged as a powerful tool for resolving cellular states associated with normal and maligned developmental processes. Here, we used scRNA-seq to examine the cell cycle states of expanding human neural stem cells (hNSCs). From these data, we constructed a cell cycle classifier that identifies traditional cell cycle phases and a putative quiescent-like state in neuroepithelial-derived cell types during mammalian neurogenesis and in gliomas. The Neural G0 markers are enriched with quiescent NSC genes and other neurodevelopmental markers found in non-dividing neural progenitors. Putative glioblastoma stem-like cells were significantly enriched in the Neural G0 cell population. Neural G0 cell populations and gene expression are significantly associated with less aMolecular systems biologyNeural G0: a quiescent-like state found in neuroepithelial-derived cells and glioma.PMC8186478T32CA080416T32 CA009657R21 CA232244P30 CA015704P30CA15704T32 CA080416R21CA1707225R21CA232244R21 CA170722CA100735R01 NS119650R01NS119650R01 CA190957R01CA190957Patel ACorrin PHoellerbauer PO'Connor SABasom RFeldman HMArora SToledo CMKufeld MDelrow JTrapnell CPaddison PJPlaisier CLMcFaline-Figueroa JLCarter LBolouri HPollard SMfalseNeural G0: a quiescent-like state found in neuroepithelial-derived cells and glioma.Single-cell RNA sequencing has emerged as a powerful tool for resolving cellular states associated with normal and maligned developmental processes. Here, we used scRNA-seq to examine the cell cycle states of expanding human neural stem cells (hNSCs). From these data, we constructed a cell cycle classifier that identifies traditional cell cycle phases and a putative quiescent-like state in neuroepithelial-derived cell types during mammalian neurogenesis and in gliomas. The Neural G0 markers are enriched with quiescent NSC genes and other neurodevelopmental markers found in non-dividing neural progenitors. Putative glioblastoma stem-like cells were significantly enriched in the Neural G0 cell population. Neural G0 cell populations and gene expression are significantly associated with less a2021-01-01T00:00:00Z2021 Jun2026-05-09T06:46:43.739Z2022-02-10T15:02:52.044ZS-EPMC81864783410135310.15252/msb.20209522