{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Chang YC"],"pubmed_abstract":["The antifungal agent 5-fluorocytosine (5-FC) is used for the treatment of several mycoses, but is unsuitable for monotherapy due to the rapid development of resistance. Here, we show that cryptococci develop resistance to 5-FC at a high frequency when exposed to concentrations several fold above the minimal inhibitory concentration. The genomes of resistant clones contain alterations in genes relevant as well as irrelevant for 5-FC resistance, suggesting that 5-FC may be mutagenic at moderate concentrations. Mutations in FCY2 (encoding a known permease for 5-FC uptake), FCY1, FUR1, UXS1 (encoding an enzyme that converts UDP-glucuronic acid to UDP-xylose) and URA6 contribute to 5-FC resistance. The uxs1 mutants accumulate UDP-glucuronic acid, which appears to down-regulate expression of per"],"journal":["Nature communications"],"pagination":["3418"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8187385"],"repository":["biostudies-literature"],"pubmed_title":["Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci."],"pmcid":["PMC8187385"],"pubmed_authors":["Walter PJ","Lamichhane AK","Bennett JE","Kwon-Chung KJ","Cai H","Chang YC"],"additional_accession":[]},"is_claimable":false,"name":"Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci.","description":"The antifungal agent 5-fluorocytosine (5-FC) is used for the treatment of several mycoses, but is unsuitable for monotherapy due to the rapid development of resistance. Here, we show that cryptococci develop resistance to 5-FC at a high frequency when exposed to concentrations several fold above the minimal inhibitory concentration. The genomes of resistant clones contain alterations in genes relevant as well as irrelevant for 5-FC resistance, suggesting that 5-FC may be mutagenic at moderate concentrations. Mutations in FCY2 (encoding a known permease for 5-FC uptake), FCY1, FUR1, UXS1 (encoding an enzyme that converts UDP-glucuronic acid to UDP-xylose) and URA6 contribute to 5-FC resistance. The uxs1 mutants accumulate UDP-glucuronic acid, which appears to down-regulate expression of per","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-21T18:09:19.167Z","creation":"2022-02-10T14:25:37.604Z"},"accession":"S-EPMC8187385","cross_references":{"pubmed":["34103502"],"doi":["10.1038/s41467-021-23745-1"]}}