{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Harrington LB"],"funding":["Howard Hughes Medical Institute","NIAID NIH HHS","NHGRI NIH HHS","National Institutes of Health","National Science Foundation"],"pagination":["416-424.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8196889"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["79(3)"],"pubmed_abstract":["CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection. However, Cas12c (C2c3)- and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observed. We show here that a short-complementarity untranslated RNA (scoutRNA), together with crRNA, is required for Cas12d-catalyzed DNA cutting. The scoutRNA differs in secondary structure from previously described tracrRNAs used by CRISPR-Cas9 and some Cas12 enzymes, and in Cas12d-containing systems, scoutRNA includes a conserved five-nucleotide sequence that is essential for activity. In addition to supporting crRNA-directed DNA recognition, biochemical and cell-based experiments establish scoutRNA as an es"],"journal":["Molecular cell"],"pubmed_title":["A scoutRNA Is Required for Some Type V CRISPR-Cas Systems."],"pmcid":["PMC8196889"],"funding_grant_id":["U01AI142817-02","R01 AI092531","U01 AI142817","1817593","RM1 HG009490"],"pubmed_authors":["Chen JS","Banfield JF","Al-Shayeb B","Doudna JA","Witte IP","Burstein D","Harrington LB","Paez-Espino D","Kyrpides NC","Ma E","Gertz D"],"additional_accession":[]},"is_claimable":false,"name":"A scoutRNA Is Required for Some Type V CRISPR-Cas Systems.","description":"CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection. However, Cas12c (C2c3)- and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observed. We show here that a short-complementarity untranslated RNA (scoutRNA), together with crRNA, is required for Cas12d-catalyzed DNA cutting. The scoutRNA differs in secondary structure from previously described tracrRNAs used by CRISPR-Cas9 and some Cas12 enzymes, and in Cas12d-containing systems, scoutRNA includes a conserved five-nucleotide sequence that is essential for activity. In addition to supporting crRNA-directed DNA recognition, biochemical and cell-based experiments establish scoutRNA as an es","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Aug","modification":"2025-04-19T00:29:08.736Z","creation":"2022-02-11T02:46:25.079Z"},"accession":"S-EPMC8196889","cross_references":{"pubmed":["32645367"],"doi":["10.1016/j.molcel.2020.06.022"]}}