biostudies-literatureYi SYGolden Seed Project , Ministry of Education of the Republic of Korea, and the National Research Foundation of Korea6017https://www.ebi.ac.uk/biostudies/studies/S-EPMC8199657biostudies-literatureUnknown22(11)Leaf senescence is a developmental process induced by various molecular and environmental stimuli that may affect crop yield. The dark-induced leaf senescence-91 (DLS-91) plants displayed rapid leaf senescence, dramatically decreased chlorophyll contents, low photochemical efficiencies, and upregulation of the senescence-associated marker gene <i>BrSAG12-1</i>. To understand DLS molecular mechanism, we examined transcriptomic changes in DLS-91 and control line DLS-42 following 0, 1, and 4 days of dark treatment (DDT) stages. We identified 501, 446, and 456 DEGs, of which 16.7%, 17.2%, and 14.4% encoded TFs, in samples from the three stages. qRT-PCR validation of 16 genes, namely, 7 MADS, 6 NAC, and 3 WRKY, suggested that <i>BrAGL8-1</i>, <i>BrAGL15-1</i>, and <i>BrWRKY70-1</i> contribute tInternational journal of molecular sciencesComparative Transcriptome-Based Mining of Senescence-Related MADS, NAC, and WRKY Transcription Factors in the Rapid-Senescence Line DLS-91 of <i>Brassica rapa</i>.PMC8199657213006-05-5-SB110, 2016R1A2B4013170 and NRF-2018K1A3A7A03089858Yi SYLu LRameneni JJSong SGLee MChoi YLee HLim YPfalseComparative Transcriptome-Based Mining of Senescence-Related MADS, NAC, and WRKY Transcription Factors in the Rapid-Senescence Line DLS-91 of <i>Brassica rapa</i>.Leaf senescence is a developmental process induced by various molecular and environmental stimuli that may affect crop yield. The dark-induced leaf senescence-91 (DLS-91) plants displayed rapid leaf senescence, dramatically decreased chlorophyll contents, low photochemical efficiencies, and upregulation of the senescence-associated marker gene <i>BrSAG12-1</i>. To understand DLS molecular mechanism, we examined transcriptomic changes in DLS-91 and control line DLS-42 following 0, 1, and 4 days of dark treatment (DDT) stages. We identified 501, 446, and 456 DEGs, of which 16.7%, 17.2%, and 14.4% encoded TFs, in samples from the three stages. qRT-PCR validation of 16 genes, namely, 7 MADS, 6 NAC, and 3 WRKY, suggested that <i>BrAGL8-1</i>, <i>BrAGL15-1</i>, and <i>BrWRKY70-1</i> contribute t2021-01-01T00:00:00Z2021 Jun2026-04-08T06:54:28.662Z2022-02-10T17:47:24.319ZS-EPMC81996573419951510.3390/ijms22116017