biostudies-literatureWu RNCCIH NIH HHSNIEHS NIH HHSNational Center for Complementary and Integrative HealthNCI NIH HHS391-402https://www.ebi.ac.uk/biostudies/studies/S-EPMC8201649biostudies-literatureUnknown60(6)Epigenetics/epigenomics has been shown to be involved in carcinogenesis. However, how the epigenome would be altered in the transgenic adenocarcinoma of the mouse prostate (TRAMP) cancer model and the effect of cancer chemopreventive phytochemical phenethyl isothiocyanate (PEITC) on the epigenome in TRAMP mice are not known. PEITC has been reported to reduce the risk of many cancers including prostate cancer (PCa). In this study, male TRAMP mice were fed a control diet or diet containing 0.05% PEITC from 8 weeks to 16 weeks. The tumor incidence was reduced in the PEITC diet (0/6) as compared with the control diet (6/7). RNA-sequencing (RNA-seq) analyses on nontumor and tumor prostatic tissues revealed several pathways like cell cycle/Cdc42 signaling, inflammation, and cancer-related signalMolecular carcinogenesisDNA methylome, transcriptome, and prostate cancer prevention by phenethyl isothiocyanate in TRAMP mice.PMC8201649R01 AT009152R01 CA200129P30 ES005022Peter RLiu XSargsyan DLi SYin RWu RKuo HCHudlikar RKong ANWang LfalseDNA methylome, transcriptome, and prostate cancer prevention by phenethyl isothiocyanate in TRAMP mice.Epigenetics/epigenomics has been shown to be involved in carcinogenesis. However, how the epigenome would be altered in the transgenic adenocarcinoma of the mouse prostate (TRAMP) cancer model and the effect of cancer chemopreventive phytochemical phenethyl isothiocyanate (PEITC) on the epigenome in TRAMP mice are not known. PEITC has been reported to reduce the risk of many cancers including prostate cancer (PCa). In this study, male TRAMP mice were fed a control diet or diet containing 0.05% PEITC from 8 weeks to 16 weeks. The tumor incidence was reduced in the PEITC diet (0/6) as compared with the control diet (6/7). RNA-sequencing (RNA-seq) analyses on nontumor and tumor prostatic tissues revealed several pathways like cell cycle/Cdc42 signaling, inflammation, and cancer-related signal2021-01-01T00:00:00Z2021 Jun2026-05-09T04:20:20.829Z2025-04-04T22:01:55.361ZS-EPMC82016493384837510.1002/mc.23299