biostudies-literatureRendeiro AFNCATS NIH HHSNIDDK NIH HHSNIAID NIH HHSNCI NIH HHS564-569https://www.ebi.ac.uk/biostudies/studies/S-EPMC8204801biostudies-literatureUnknown593(7860)Recent studies have provided insights into the pathology of and immune response to COVID-19^1-8. However, a thorough investigation of the interplay between infected cells and the immune system at sites of infection has been lacking. Here we use high-parameter imaging mass cytometry⁹ that targets the expression of 36 proteins to investigate the cellular composition and spatial architecture of acute lung injury in humans (including injuries derived from SARS-CoV-2 infection) at single-cell resolution. These spatially resolved single-cell data unravel the disordered structure of the infected and injured lung, alongside the distribution of extensive immune infiltration. Neutrophil and macrophage infiltration are hallmarks of bacterial pneumonia and COVID-19, respectively. NatureThe spatial landscape of lung pathology during COVID-19 progression.PMC8204801R01 AI107301R01 DK121072UL1 TR002384R01 CA234614R01 CA194547T32 CA203702R03 DK117252Bram YReeves JPark JElemento OMason CEWarren SRendeiro AFSalvatore SMeydan CKim JBorczuk ACHether TSwanson ECSchwartz REChandar VKim YRavichandran HFoox JfalseThe spatial landscape of lung pathology during COVID-19 progression.Recent studies have provided insights into the pathology of and immune response to COVID-19^1-8. However, a thorough investigation of the interplay between infected cells and the immune system at sites of infection has been lacking. Here we use high-parameter imaging mass cytometry⁹ that targets the expression of 36 proteins to investigate the cellular composition and spatial architecture of acute lung injury in humans (including injuries derived from SARS-CoV-2 infection) at single-cell resolution. These spatially resolved single-cell data unravel the disordered structure of the infected and injured lung, alongside the distribution of extensive immune infiltration. Neutrophil and macrophage infiltration are hallmarks of bacterial pneumonia and COVID-19, respectively. 2021-01-01T00:00:00Z2021 May2026-04-08T09:10:33.712Z2022-02-11T12:22:43.474ZS-EPMC82048013378096910.1038/s41586-021-03475-6