{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ordureau A"],"funding":["NHGRI NIH HHS","NINDS NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["3009-3013"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8210946"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(5)"],"pubmed_abstract":["Stable isotope labeling is a leading strategy for mass-spectrometry-based peptide quantification. Whereas TMTpro isobaric tagging can quantify up to 16 multiplexed samples in a single experiment, nonisobaric, yet chromatographically indistinguishable, variants of TMTpro reagents can be used in conjunction with the isobaric tag series for various peptide-targeting applications. Here we test the performance of two nonisobaric TMTpro variants, a stable-isotope-free TMTproZero tag and a nearly fully isotope-labeled \"super-heavy\" variant, shTMTpro, in a targeted assay for peptides of charge state 4+. We label each peptide with TMTproZero or Super Heavy TMTpro reagents and separately spike each peptide into a TMTpro16-labeled background (equal amount of peptide across all 16 channels). We observ"],"journal":["Journal of proteome research"],"pubmed_title":["Super Heavy TMTpro Labeling Reagent: An Alternative and Higher-Charge-State-Amenable Stable-Isotope-Labeled TMTpro Variant."],"pmcid":["PMC8210946"],"funding_grant_id":["NS083524-11","R01 GM132129","U24 HG006673","GM67945","R01 NS083524","GM132129","R37 NS083524","HG006673-07","U41 HG006673","R01 GM067945"],"pubmed_authors":["Harper JW","Paulo JA","Yu Q","Rogers JC","Gygi SP","Ordureau A","Bomgarden RD"],"additional_accession":[]},"is_claimable":false,"name":"Super Heavy TMTpro Labeling Reagent: An Alternative and Higher-Charge-State-Amenable Stable-Isotope-Labeled TMTpro Variant.","description":"Stable isotope labeling is a leading strategy for mass-spectrometry-based peptide quantification. Whereas TMTpro isobaric tagging can quantify up to 16 multiplexed samples in a single experiment, nonisobaric, yet chromatographically indistinguishable, variants of TMTpro reagents can be used in conjunction with the isobaric tag series for various peptide-targeting applications. Here we test the performance of two nonisobaric TMTpro variants, a stable-isotope-free TMTproZero tag and a nearly fully isotope-labeled \"super-heavy\" variant, shTMTpro, in a targeted assay for peptides of charge state 4+. We label each peptide with TMTproZero or Super Heavy TMTpro reagents and separately spike each peptide into a TMTpro16-labeled background (equal amount of peptide across all 16 channels). We observ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2025-04-05T14:05:41.26Z","creation":"2025-04-05T14:05:41.26Z"},"accession":"S-EPMC8210946","cross_references":{"pubmed":["33689365"],"doi":["10.1021/acs.jproteome.0c01056"]}}