{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang C"],"funding":["National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["6364-6374"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8216280"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["49(11)"],"pubmed_abstract":["DNA-hydrolyzing DNAs represent an attractive type of DNA-processing catalysts distinctive from the protein-based restriction enzymes. The innate DNA property has enabled them to readily join DNA-based manipulations to promote the development of DNA biotechnology. A major in vitro selection strategy to identify these DNA catalysts relies tightly on the isolation of linear DNAs processed from a circular single-stranded (ss) DNA sequence library by self-hydrolysis. Herein, we report that by programming a terminal hybridization stem in the library, other than the previously reported classes (I & II) of deoxyribozymes, two new classes (III & IV) were identified with the old selection strategy to site-specifically hydrolyze DNA in the presence of Zn2+. Their representatives own a catalytic core "],"journal":["Nucleic acids research"],"pubmed_title":["New DNA-hydrolyzing DNAs isolated from an ssDNA library carrying a terminal hybridization stem."],"pmcid":["PMC8216280"],"funding_grant_id":["21673050","91859104","81861138004","2020YFA0908901"],"pubmed_authors":["Li W","He Y","Gu H","Xu T","Li Q","Zhang C"],"additional_accession":[]},"is_claimable":false,"name":"New DNA-hydrolyzing DNAs isolated from an ssDNA library carrying a terminal hybridization stem.","description":"DNA-hydrolyzing DNAs represent an attractive type of DNA-processing catalysts distinctive from the protein-based restriction enzymes. The innate DNA property has enabled them to readily join DNA-based manipulations to promote the development of DNA biotechnology. A major in vitro selection strategy to identify these DNA catalysts relies tightly on the isolation of linear DNAs processed from a circular single-stranded (ss) DNA sequence library by self-hydrolysis. Herein, we report that by programming a terminal hybridization stem in the library, other than the previously reported classes (I & II) of deoxyribozymes, two new classes (III & IV) were identified with the old selection strategy to site-specifically hydrolyze DNA in the presence of Zn2+. Their representatives own a catalytic core ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-04T20:18:19.464Z","creation":"2022-02-10T15:47:17.448Z"},"accession":"S-EPMC8216280","cross_references":{"pubmed":["34057476"],"doi":["10.1093/nar/gkab439"]}}