biostudies-literatureLiang BNIDDK NIH HHSBurroughs Wellcome FundNCI NIH HHSNational Institutes of Health120-131https://www.ebi.ac.uk/biostudies/studies/S-EPMC8217095biostudies-literatureUnknown75(1)<h4>Background & aims</h4>Gain of function (GOF) mutations in the CTNNB1 gene are one of the most frequent genetic events in hepatocellular carcinoma (HCC). T-box transcription factor 3 (TBX3) is a liver-specific target of the Wnt/β-catenin pathway and thought to be an oncogene mediating activated β-catenin-driven HCC formation.<h4>Methods</h4>We evaluated the expression pattern of TBX3 in human HCC specimens. Tbx3 was conditionally knocked out in murine HCC models by hydrodynamic tail vein injection of Cre together with c-Met and ΔN90-β-catenin (c-Met/β-catenin) in Tbx3^flox/flox mice. TBX3 was overexpressed in human HCC cell lines to investigate the functions of TBX3 in vitro.<h4>Results</h4>A bimodal expression pattern of TBX3 in human HCC samples was detected: high expressionJournal of hepatologyTBX3 functions as a tumor suppressor downstream of activated CTNNB1 mutants during hepatocarcinogenesis.PMC8217095P30 DK026743P30 DK120531P30DK026743R01 CA251155R01 CA239251R01CA239251R01 CA250227K08DK101603R01CA204586R01CA250227K08 DK101603R01 CA204586Xu MLin SWang BWang HZhou YMonga SPWang JRen CEvert MLiang BHuang ZZhang YChen YChen XSong XCalvisi DFQian MfalseTBX3 functions as a tumor suppressor downstream of activated CTNNB1 mutants during hepatocarcinogenesis.<h4>Background & aims</h4>Gain of function (GOF) mutations in the CTNNB1 gene are one of the most frequent genetic events in hepatocellular carcinoma (HCC). T-box transcription factor 3 (TBX3) is a liver-specific target of the Wnt/β-catenin pathway and thought to be an oncogene mediating activated β-catenin-driven HCC formation.<h4>Methods</h4>We evaluated the expression pattern of TBX3 in human HCC specimens. Tbx3 was conditionally knocked out in murine HCC models by hydrodynamic tail vein injection of Cre together with c-Met and ΔN90-β-catenin (c-Met/β-catenin) in Tbx3^flox/flox mice. TBX3 was overexpressed in human HCC cell lines to investigate the functions of TBX3 in vitro.<h4>Results</h4>A bimodal expression pattern of TBX3 in human HCC samples was detected: high expression2021-01-01T00:00:00Z2021 Jul2026-05-08T01:13:07.428Z2022-07-10T09:34:20.069ZS-EPMC82170953357792110.1016/j.jhep.2021.01.044