{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Eichholz K"],"funding":["National institute of health","NIAID NIH HHS","NCI NIH HHS","Gilead Sciences"],"pagination":["12921"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8217486"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Chimeric antigen receptor (CAR) T cells are engineered cells used in cancer therapy and are studied to treat infectious diseases. Trafficking and persistence of CAR T cells is an important requirement for efficacy to target cancer. Here, we describe a CAR RNA FISH histo-cytometry platform combined with a random reaction seed image analysis algorithm to quantitate spatial distribution and in vivo functional activity of a CAR T cell population at a single cell resolution for preclinical models. In situ, CAR T cell exhibited a heterogenous effector gene expression and this was related to the distance from tumor cells, allowing a quantitative assessment of the potential in vivo effectiveness. The platform offers the potential to study immune functions of genetically engineered cells in situ wi"],"journal":["Scientific reports"],"pubmed_title":["A CAR RNA FISH assay to study functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue."],"pmcid":["PMC8217486"],"funding_grant_id":["R01 AI143773","P30 CA015704","HIV Cure Grant Program","UM1 AI126623","UM1 A126623"],"pubmed_authors":["Li AZ","Zhu J","Jensen MC","Corey L","Diem K","Eichholz K"],"additional_accession":[]},"is_claimable":false,"name":"A CAR RNA FISH assay to study functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue.","description":"Chimeric antigen receptor (CAR) T cells are engineered cells used in cancer therapy and are studied to treat infectious diseases. Trafficking and persistence of CAR T cells is an important requirement for efficacy to target cancer. Here, we describe a CAR RNA FISH histo-cytometry platform combined with a random reaction seed image analysis algorithm to quantitate spatial distribution and in vivo functional activity of a CAR T cell population at a single cell resolution for preclinical models. In situ, CAR T cell exhibited a heterogenous effector gene expression and this was related to the distance from tumor cells, allowing a quantitative assessment of the potential in vivo effectiveness. The platform offers the potential to study immune functions of genetically engineered cells in situ wi","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2026-05-08T18:57:48.133Z","creation":"2022-02-11T11:00:20.324Z"},"accession":"S-EPMC8217486","cross_references":{"pubmed":["34155235"],"doi":["10.1038/s41598-021-92196-x"]}}