{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Roncato F"],"funding":["German-Israeli Foundation for Scientific Research and Development"],"pagination":["166-179"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8218694"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Microtubules (MTs) control cell shape and intracellular cargo transport. The role of MT turnover in the migration of slow-moving cells through endothelial barriers remains unclear. To irreversibly interfere with MT disassembly, we have used the MT-stabilizing agent zampanolide (ZMP) in Β16F10 melanoma as amodel of slow-moving cells. ZMP-treated B16 cells failed to follow chemotactic gradients across rigid confinements and could not generate stable sub-endothelial pseudopodia under endothelial monolayers. In vivo, ZMP-treated Β16 cells failed to extravasate though lung capillaries. In contrast to melanoma cells, the chemotaxis and transendothelial migration of ZMP-treated Tcells were largely conserved. This is afirst demonstration that MT disassembly is akey checkpoint in the directional mi"],"journal":["Cell adhesion & migration"],"pubmed_title":["Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells."],"pmcid":["PMC8218694"],"funding_grant_id":["[I-1470-412.13/2018]"],"pubmed_authors":["Yadav SK","Regev O","Roncato F","Alon R"],"additional_accession":[]},"is_claimable":false,"name":"Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells.","description":"Microtubules (MTs) control cell shape and intracellular cargo transport. The role of MT turnover in the migration of slow-moving cells through endothelial barriers remains unclear. To irreversibly interfere with MT disassembly, we have used the MT-stabilizing agent zampanolide (ZMP) in Β16F10 melanoma as amodel of slow-moving cells. ZMP-treated B16 cells failed to follow chemotactic gradients across rigid confinements and could not generate stable sub-endothelial pseudopodia under endothelial monolayers. In vivo, ZMP-treated Β16 cells failed to extravasate though lung capillaries. In contrast to melanoma cells, the chemotaxis and transendothelial migration of ZMP-treated Tcells were largely conserved. This is afirst demonstration that MT disassembly is akey checkpoint in the directional mi","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T10:53:24.992Z","creation":"2022-02-10T17:01:05.336Z"},"accession":"S-EPMC8218694","cross_references":{"pubmed":["34152257"],"doi":["10.1080/19336918.2021.1934958"]}}