biostudies-literatureRoncato FGerman-Israeli Foundation for Scientific Research and Development166-179https://www.ebi.ac.uk/biostudies/studies/S-EPMC8218694biostudies-literatureUnknown15(1)Microtubules (MTs) control cell shape and intracellular cargo transport. The role of MT turnover in the migration of slow-moving cells through endothelial barriers remains unclear. To irreversibly interfere with MT disassembly, we have used the MT-stabilizing agent zampanolide (ZMP) in Β16F10 melanoma as amodel of slow-moving cells. ZMP-treated B16 cells failed to follow chemotactic gradients across rigid confinements and could not generate stable sub-endothelial pseudopodia under endothelial monolayers. In vivo, ZMP-treated Β16 cells failed to extravasate though lung capillaries. In contrast to melanoma cells, the chemotaxis and transendothelial migration of ZMP-treated Tcells were largely conserved. This is afirst demonstration that MT disassembly is akey checkpoint in the directional miCell adhesion & migrationMicrotubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells.PMC8218694[I-1470-412.13/2018]Yadav SKRegev ORoncato FAlon RfalseMicrotubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells.Microtubules (MTs) control cell shape and intracellular cargo transport. The role of MT turnover in the migration of slow-moving cells through endothelial barriers remains unclear. To irreversibly interfere with MT disassembly, we have used the MT-stabilizing agent zampanolide (ZMP) in Β16F10 melanoma as amodel of slow-moving cells. ZMP-treated B16 cells failed to follow chemotactic gradients across rigid confinements and could not generate stable sub-endothelial pseudopodia under endothelial monolayers. In vivo, ZMP-treated Β16 cells failed to extravasate though lung capillaries. In contrast to melanoma cells, the chemotaxis and transendothelial migration of ZMP-treated Tcells were largely conserved. This is afirst demonstration that MT disassembly is akey checkpoint in the directional mi2021-01-01T00:00:00Z2021 Dec2025-04-04T10:53:24.992Z2022-02-10T17:01:05.336ZS-EPMC82186943415225710.1080/19336918.2021.1934958