biostudies-literatureUnknownCourson JASerial block-face scanning electron microscopy (SBF-SEM) allows for the collection of hundreds to thousands of serially-registered ultrastructural images, offering an unprecedented three-dimensional view of tissue microanatomy. While SBF-SEM has seen an exponential increase in use in recent years, technical aspects such as proper tissue preparation and imaging parameters are paramount for the success of this imaging modality. This imaging system benefits from the automated nature of the device, allowing one to leave the microscope unattended during the imaging process, with the automated collection of hundreds of images possible in a single day. However, without appropriate tissue preparation cellular ultrastructure can be altered in such a way that incorrect or misleading conclusions mighJournal of visualized experiments : JoVEhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC8225236biostudies-literatureSerial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples.PMC8225236Rumbaut RELandry PTPatel NLafontant PJSpehlmann EBurns ARCourson JADo TfalseSerial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples.Serial block-face scanning electron microscopy (SBF-SEM) allows for the collection of hundreds to thousands of serially-registered ultrastructural images, offering an unprecedented three-dimensional view of tissue microanatomy. While SBF-SEM has seen an exponential increase in use in recent years, technical aspects such as proper tissue preparation and imaging parameters are paramount for the success of this imaging modality. This imaging system benefits from the automated nature of the device, allowing one to leave the microscope unattended during the imaging process, with the automated collection of hundreds of images possible in a single day. However, without appropriate tissue preparation cellular ultrastructure can be altered in such a way that incorrect or misleading conclusions migh2021-01-01T00:00:00Z2021 Mar2025-04-05T10:03:41.338Z2025-04-05T10:03:41.338ZS-EPMC82252363384393110.3791/62045