biostudies-literatureUnknown11(6)Ambrosio GEcdysterone is a phytosteroid widely discussed for its various pharmacological, growth-promoting, and anabolic effects, mediated by the activation of estrogen receptor beta (ERbeta). Performance-enhancement in sports was demonstrated recently, and ecdysterone was consequently included in the Monitoring Program, to detect potential patterns of misuse in sport. Only few studies on the pharmacokinetics of ecdysterone in humans have been reported so far. In this study, post-administration urine samples in twelve volunteers (single dose of 50 mg of ecdysterone) were analyzed using dilute-and-inject liquid-chromatography-tandem mass spectrometry. Identification and quantitation of ecdysterone and of two metabolites, 14-deoxy-ecdysterone and 14-deoxy-poststerone, was achieved. Ecdysterone was theMetabolites366https://www.ebi.ac.uk/biostudies/studies/S-EPMC8227119biostudies-literatureUrinary Elimination of Ecdysterone and Its Metabolites Following a Single-Dose Administration in Humans.PMC8227119Wuest BIsenmann EYuliandra TMazzarino MDiel Pde la Torre XBotre FParr MKAmbrosio GfalseUrinary Elimination of Ecdysterone and Its Metabolites Following a Single-Dose Administration in Humans.Ecdysterone is a phytosteroid widely discussed for its various pharmacological, growth-promoting, and anabolic effects, mediated by the activation of estrogen receptor beta (ERbeta). Performance-enhancement in sports was demonstrated recently, and ecdysterone was consequently included in the Monitoring Program, to detect potential patterns of misuse in sport. Only few studies on the pharmacokinetics of ecdysterone in humans have been reported so far. In this study, post-administration urine samples in twelve volunteers (single dose of 50 mg of ecdysterone) were analyzed using dilute-and-inject liquid-chromatography-tandem mass spectrometry. Identification and quantitation of ecdysterone and of two metabolites, 14-deoxy-ecdysterone and 14-deoxy-poststerone, was achieved. Ecdysterone was the2021-01-01T00:00:00Z2021 Jun2025-05-31T23:33:27.487Z2025-05-31T23:33:27.487ZS-EPMC82271193420756910.3390/metabo11060366